An Environmental Shiga Toxin-Producing Escherichia coli O145 Clonal Population Exhibits High-Level Phenotypic Variation That Includes Virulence Traits

Applied and Environmental Microbiology
Michelle Qiu CarterMichael B Cooley

Abstract

Shiga toxin-producing Escherichia coli (STEC) serotype O145 is one of the major non-O157 serotypes associated with severe human disease. Here we examined the genetic diversity, population structure, virulence potential, and antimicrobial resistance profiles of environmental O145 strains recovered from a major produce production region in California. Multilocus sequence typing analyses revealed that sequence type 78 (ST-78), a common ST in clinical strains, was the predominant genotype among the environmental strains. Similarly, all California environmental strains belonged to H28, a common H serotype in clinical strains. Although most environmental strains carried an intact fliC gene, only one strain retained swimming motility. Diverse stx subtypes were identified, including stx1a, stx2a, stx2c, and stx2e. Although no correlation was detected between the stx genotype and Stx1 production, high Stx2 production was detected mainly in strains carrying stx2a only and was correlated positively with the cytotoxicity of Shiga toxin. All environmental strains were capable of producing enterohemolysin, whereas only 10 strains were positive for anaerobic hemolytic activity. Multidrug resistance appeared to be common, as nearly half of the...Continue Reading

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Citations

Dec 7, 2016·Trends in Microbiology·Michelle Qiu Carter
Mar 9, 2019·Foodborne Pathogens and Disease·Michelle Qiu CarterMichael B Cooley
Apr 21, 2018·Genome Announcements·Michelle Qiu Carter, Antares Pham
May 12, 2018·Genome Announcements·Michelle Qiu Carter, Antares Pham
Jul 3, 2021·Animals : an Open Access Journal From MDPI·Nicolás GalarceNilton Lincopan

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