PMID: 7035568Jan 1, 1982Paper

An enzymatic method for preparation of dissociated murine Peyer's patch cells enriched for macrophages

Journal of Immunological Methods
M V FrangakisJ R McGhee

Abstract

Enzyme (Dispase) dissociation of murine Peyer's patches (PP) 3 yields a cell population rich in T and B lymphocytes and containing 5-9% macrophages (MO) as defined by morphology and esterase staining. Cell yields by this method are 3-4 times greater than with conventional mechanical methods and viability of cells always exceeds 95%. The virtual absence of IgA plasma cells (less than 1%) indicates lack of contamination of the cell preparations with intestinal mucosal cells. Enzymatically dissociated PP cells proliferate in response to both T and B cell mitogens. Differences in patterns of mitogen responsiveness of sequentially obtained PP cell fractions are observed suggesting considerable functional heterogeneity of PP cells. Patterns of IgM and IgA synthesis by PP cells in response to the B cell polyclonal activator LPS differ considerably from spleen cells. The development of methodology for preparation of PP cells containing functionally intact T and B cells and enriched for MO should permit analysis of the cellular basis for functional specialization of PP.

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Citations

Jul 1, 1989·Journal of Clinical Immunology·H Z HirschJ Mestecky
Apr 2, 1993·Journal of Immunological Methods·K FujihashiH Kiyono
Jun 1, 1990·Journal of Reproductive Immunology·S Kamel, G W Wood
Aug 1, 1989·Veterinary Immunology and Immunopathology·A M Nagi, L A Babiuk
Oct 1, 1995·Veterinary Immunology and Immunopathology·T MurayamaK Kaneko
May 1, 1996·Journal of Neuroimmunology·K A Kelly, C C Whitacre
Dec 11, 2002·Cellular Immunology·Ji Hee ParkPyeung Hyeun Kim
Sep 1, 1984·The Journal of Experimental Medicine·D M SpaldingJ R McGhee
Jan 1, 1988·Acta Pathologica Japonica·S Kawaguchi
Jan 1, 1986·Infection·C De SimoneF Sorice
Apr 24, 2013·Animal Science Journal = Nihon Chikusan Gakkaihō·Yasuhiro IharaFumiki Morimatsu
Jun 21, 2011·Biochemical and Biophysical Research Communications·Mi-Ra LeePyeung-Hyeun Kim
Sep 1, 1988·Clinical Immunology and Immunopathology·S KawaguchiY Masugi
Nov 1, 1988·Journal of Oral Pathology·H Z HirschJ Mestecky
Mar 15, 2006·Immunobiology·Gabriel VinderolaChantal Matar
Jan 1, 1988·Immunopharmacology and Immunotoxicology·C De SimoneR Vesely
Dec 3, 2014·Vaccine·Aayam LamichhaneHiroshi Kiyonoa
Jan 1, 1985·Scandinavian Journal of Gastroenterology. Supplement·C O Elson
Jun 1, 1989·European Journal of Immunology·S SchoenbeckM F Kagnoff
Jun 30, 1983·Annals of the New York Academy of Sciences·W J KoopmanR E Schrohenloher
Jan 27, 2009·Journal of Leukocyte Biology·Goo-Young SeoPyeung-Hyeun Kim
Dec 1, 1984·Neurochemical Research·M V Frangakis, H K Kimelberg
May 23, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M MoriF D Finkelman

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