PMID: 6403623Mar 11, 1983Paper

An enzyme immunofiltration assay useful for detecting human monoclonal antibody

Journal of Immunological Methods
M C GlassyI Royston

Abstract

A microenzyme-linked immunoassay (EIA) utilizing an immunofiltration manifold has been developed which provides a rapid, simple, and sensitive method of detecting human monoclonal antibody class, concentration, and specificity. In this assay either whole cells or soluble antigens were immobilized on glass fiber filters followed by incubating with the test human hybridoma supernatant with subsequent analysis by EIA. A specially designed 96-chamber immunofiltration plate is employed which serves as both an incubation chamber and as a filtration manifold. The assay described is unique in that small volumes of human hybridoma supernatant are required, crude preparation of only a few target cells are needed, labile cell surface antigens are preserved and it can be completed in 3 h. This assay is well suited for the rapid screening of large numbers of human hybridoma supernatants.

References

Mar 1, 1977·Somatic Cell Genetics·M L GefterM D Scharff
Jan 1, 1976·Journal of Immunological Methods·J E Herrmann, M F Collins
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M C GlassyI Royston

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Citations

Nov 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M C GlassyA Mikhalev
Jul 16, 1985·Journal of Immunological Methods·M C Glassy, C D Surh
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M C GlassyI Royston
May 1, 1986·Forensic Science International : Synergy·T Takatori, Y Tsutsubuchi
Jan 1, 1987·Cancer Investigation·M C GlassyI Royston
Oct 1, 1984·The Journal of Urology·D H LoweM C Glassy

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