An enzyme-linked immunosorbent assay (ELISA) for the determination of mucin levels in bronchoalveolar lavage fluid

Journal of Pharmacological and Toxicological Methods
Jonathan E PhillipsMichael Minnicozzi

Abstract

A method to measure the mucin concentration in bronchoalveolar lavage (BAL) fluid was developed to aid efforts to identify pharmacologically the mechanisms that modulate pathophysiological mucin secretion. Mucins are the major macromolecular components of mucus. In the airways, mucus is the first line of defense against inhaled microorganisms (infection) and particulates (irritation). An enzyme-linked immunosorbent assay (ELISA) was developed, comparing two monoclonal anti-mucin antibodies (A10G5 and 45M1) raised to human mucin, to quantify the mucin in BAL fluid from animal models of pulmonary inflammation. To validate the ELISA method, rats were exposed to ovalbumin (OVA, in sensitized rats), lipopolysaccharide (LPS), vanadium pentoxide (V(2)O(5)), or saline. One hundred microliters of BAL fluid was analyzed for mucin concentration. Pooled BAL fluid from untreated rats was used as an internal "plate standard", as a standard mucin that cross-reacts with A10G5 was unavailable. We found both antibodies reacted with rat, human, and guinea-pig mucin; where the 45M1 antibody also reacted with the mucin in porcine BAL, while A10G5 did not. We determined the mucin concentration in each BAL fluid sample relative to the standard, defin...Continue Reading

References

Jan 21, 1991·International Journal of Cancer. Journal International Du Cancer·J BaraN Daher
Jul 1, 1989·American Journal of Respiratory Cell and Molecular Biology·H LinR Wu
Dec 1, 1995·American Journal of Respiratory Cell and Molecular Biology·J K SheehanD J Thornton
May 1, 1994·American Journal of Respiratory Cell and Molecular Biology·D SteigerC Basbaum
May 1, 1993·The American Review of Respiratory Disease·J V FahyH A Boushey
Oct 1, 1996·Glycoconjugate Journal·H W HovenbergI Carlstedt
Sep 17, 1998·The Journal of Clinical Investigation·H MatsuiR C Boucher
Dec 5, 1998·Mediators of Inflammation·H Sossé-AlaouiC Brink
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·K TakeyamaJ A Nadel
Jan 19, 2000·Toxicology and Applied Pharmacology·M LongphreC B Basbaum
Nov 9, 2000·American Journal of Respiratory and Critical Care Medicine·B T Chen, D B Yeates
Mar 29, 2001·Journal of Anatomy·J H WiddicombeS J Bastacky
Mar 29, 2001·Pulmonary Pharmacology & Therapeutics·J SpondM Minnicozzi
Aug 21, 2001·The European Respiratory Journal·P RogerC Brink
Oct 2, 2001·Frontiers in Bioscience : a Journal and Virtual Library·A P CorfieldC S Probert
Nov 20, 2001·American Journal of Respiratory Cell and Molecular Biology·M C RoseJ A Voynow
May 7, 2002·International Journal of Cancer. Journal International Du Cancer·Séverine NolletJacques Bara
Aug 10, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kazuhiro KohriJay A Nadel
Jul 10, 2003·Pulmonary Pharmacology & Therapeutics·J SpondM Minnicozzi
Jul 29, 2003·American Journal of Respiratory Cell and Molecular Biology·Yin ChenReen Wu
Dec 18, 2003·Hybridoma and Hybridomics·K RousseauD M Swallow
Feb 28, 2004·American Journal of Respiratory Cell and Molecular Biology·Markus O HenkeBruce K Rubin
Mar 17, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Je Hyeong KimSe Hwa Yoo
May 5, 2004·Pulmonary Pharmacology & Therapeutics·J SpondM Minnicozzi
Jun 29, 2004·Treatments in Respiratory Medicine·Suil Kim, Jay A Nadel
Jul 3, 2004·American Journal of Respiratory Cell and Molecular Biology·Philip A KempAlan D Jackson

❮ Previous
Next ❯

Citations

May 3, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Dongfang YuYin Chen
Jan 11, 2012·Antimicrobial Agents and Chemotherapy·David L MacLeodWilliam R Baker
Feb 28, 2018·Frontiers in Immunology·Helen T GrovesJohn S Tregoning
May 3, 2012·MBio·Amy T Y YeungRobert E W Hancock
Sep 30, 2010·The Journal of Pharmacology and Experimental Therapeutics·S LuJ M Farley
Jun 15, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·W Michael FosterJudith A Voynow

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved