PMID: 6403627Mar 25, 1983Paper

An enzyme-linked immunosorbent assay (ELISA) for the detection of monoclonal antibodies recognizing surface antigens expressed on viable cells

Journal of Immunological Methods
C FeitY Hirshaut

Abstract

A solid-phase enzyme-linked immunosorbent assay (ELISA) has been developed for detecting monoclonal antibodies binding to surface antigens expressed on viable adherent cells of tumor cell lines. This assay utilizes a sheep anti-mouse IgG to which a beta-galactosidase is linked. It is highly sensitive and permits quantification of IgG monoclonal antibody levels. In studies of monoclonal antibodies prepared against human tumors, the ELISA assay revealed the presence of antigens which were not seen using acetone-fixed cell immunofluorescence methods. This assay is safe, rapid, low cost, and gives reproducible quantitative results. As such it should prove useful to laboratories engaged in the study of antigens expressed on cell membranes.

References

Aug 3, 1973·Science·B Mukherji, Y Hirshaut
Nov 21, 1974·The New England Journal of Medicine·Y HirshautE Essner
Jan 1, 1981·Journal of Immunological Methods·D BuchananN H Ruddle

❮ Previous
Next ❯

Citations

Jan 1, 1986·Medical Microbiology and Immunology·A H BartalY Hirshaut
Jan 21, 1985·Journal of Immunological Methods·S L Epstein, J K Lunney
May 1, 1986·Journal of Immunological Methods·M Lefroit-Joliy, T Neveu
Jan 21, 1992·Journal of Immunological Methods·C d'Uscio, K Blaser
Oct 31, 2001·Journal of Immunological Methods·L F ErdileD Berd
Feb 12, 2000·Journal of Immunological Methods·Z LiuH von Grafenstein
Jan 1, 1988·Journal of Immunoassay·M BrunnerS Chin
Feb 1, 1987·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·E LavieA H Bartal
May 14, 1993·Cancer Letters·N K GuptaC Feit
Apr 25, 2008·Current Protocols in Immunology·P Hornbeck
Jan 1, 1987·Critical Reviews in Clinical Laboratory Sciences·P J MoldofskyN D Hammond
Aug 4, 2015·Current Protocols in Immunology·Peter V Hornbeck
Feb 12, 2008·Current Protocols in Molecular Biology·P HornbeckS A Fuller

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.