An enzyme-linked immunosorbent assay (ELISA) for quantification of antibodies to Streptococcus mutans surface antigens

Molecular Immunology
S HamadaJ R McGhee

Abstract

An ELISA was developed to quantitate the level of antibodies to various cell surface antigens of the Gram positive bacterium, Streptococcus mutans. Whole cells and purified cell wall components of S. mutans, lipoteichoic acid (LTA) from Streptococcus pyogenes, and dextran T 2000 were employed as coating antigens in this study. Cell walls of S. mutans were purified by mechanical disruption of whole cells followed by differential centrifugation and proteolytic enzyme treatment. Serotype-specific carbohydrate was purified from an autoclaved, lyophilized S. mutans whole cell preparation by column chromatography. LTA was prepared by Sepharose 4B chromatography of a phenol-water extract of S. pyogenes and used for detection of anti-polyglycerophosphate (PGP) antibodies. A rabbit antiserum to S. mutans 6715 (serotype g), which precipitated with purified carbohydrate antigen (RR g), gave good reactions with purified cell walls and whole cells of S. mutans 6715, less activity with RR g and low activity to LTA and dextran when tested by ELISA. Adsorption of this antiserum with whole cells of S. pyogenes resulted in antibody activity with specificity only to the serotype carbohydrate. The specificity of the antibody for homologous coating...Continue Reading

References

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Citations

Jan 1, 1985·Journal of Clinical Immunology·R L GregoryJ R McGhee
May 1, 1987·The Science of the Total Environment·G E Smith
May 9, 2002·International Journal of Pediatric Otorhinolaryngology·Yuji Yokoyama, Yasuaki Harabuchi
Mar 4, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mina RyuChangJu Chun

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