PMID: 6403665Jan 1, 1983Paper

An enzyme-linked immunosorbent assay for an antigen related to non-A, non-B hepatitis and its antibody: partial characterization of the antigen and chimpanzee transmission

Journal of Medical Virology
W DuermeyerJ A Hellings

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed based on sera from patients convalescent from non-A, non-B hepatitis and haemophilia A patients who had been frequently treated with commercial blood products. Using this ELISA, an antigen was detected which appears to be related to non-A, non-B hepatitis. The antigen is provisionally designated as DS-antigen (DS-Ag). The serum of another patient with haemophilia A, which was strongly positive for the DS-Ag, caused a typical case of non-A, non-B hepatitis in a chimpanzee. DS-Ag could be detected in the serum of the chimpanzee during the acute phase of the infection. The ELISA for DS-Ag reacted with neither hepatitis A or B virus antigens, nor Epstein-Barr virus or cytomegalovirus. The assay was provisionally evaluated using sera from different groups of patients. Out of 17 patients with posttransfusion hepatitis non-A, non-B, 11 were found positive in the ELISA for DS-Ag (65%). As expected, a relatively high prevalence of DS-Ag (9%) was found in patients with haemophilia, who are regularly treated with blood-clotting factor-concentrates. Antibodies to DS-Ag were found in 48% of these patients. The DS-Ag was found in 8 of 1400 (0.6%) volunteer blood donors, and antibody ...Continue Reading

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Citations

May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K H BurkG R Dreesman
Jan 1, 1989·Journal of Medical Virology·J B ZeldisH Steinberg
Apr 1, 1985·Journal of Medical Virology·J TohmatsuT Shikata
Dec 24, 2013·Journal of Molecular and Cellular Cardiology·Leena KaikkonenRisto Kerkelä
Mar 1, 1985·Journal of Medical Virology·M RoggendorfE Tabor
Jan 1, 1994·Vox Sanguinis·A M Prince

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