PMID: 8469912Apr 1, 1993Paper

An enzyme-linked immunosorbent assay for plasma-lactoferrin. Concentrations in 362 healthy, adult blood donors

Scandinavian Journal of Clinical and Laboratory Investigation
S AntonsenO Blaabjerg

Abstract

Very different concentrations of plasma-lactoferrin in healthy adults have been reported in the literature. We compared three commercially available lactoferrins and lactoferrin purified in our laboratory as calibrators in an ELISA. No statistical differences among these preparations of lactoferrin were detected. The concentration of purified lactoferrin was measured by dry weight, and efforts were made in order to minimize loss of purified lactoferrin by adhesion to tubes etc. and thus, secure accuracy of the method. Dilutions were made in PBS 0.01 mol l-1 with NaCl 0.436 mol l-1, (NH4)2SO4 0.5 mol l-1, BSA 5 gl-1 and normal rabbit IgG 10 mg l-1, which was shown to give parallel dilution curves of primary calibrator, secondary calibrator and plasma samples. No significant difference in the content of lactoferrin in neutrophils (median; range) among men (1.78; 0.83-4.48 micrograms 10(-6) neutrophils; n = 20) and women (2.12; 1.16-9.30 micrograms 10(-6) neutrophils; n = 14) was found. Lactoferrin was analysed in EDTA-plasma obtained from 135 female and 227 male blood donors. Median concentrations were 84.7 and 97.8 micrograms l-1 respectively, while 2.5% and 97.5% reference limits (with 90% confidence intervals) were estimated t...Continue Reading

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Citations

Sep 14, 2001·Oral Microbiology and Immunology·A L LinC K Yeh
Oct 26, 1999·Viral Immunology·B W van der StrateP J Swart
May 13, 1998·Archives of Disease in Childhood. Fetal and Neonatal Edition·M NyboP Wang
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Jan 1, 1995·Scandinavian Journal of Infectious Diseases·B SöderquistT Vikerfors
Apr 1, 1993·Scandinavian Journal of Clinical and Laboratory Investigation·S Antonsen, M Wanscher
May 1, 1993·Scandinavian Journal of Clinical and Laboratory Investigation·S AntonsenM Wanscher

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