An enzyme-linked immunosorbent assay for rare minnow (Gobiocypris rarus) vitellogenin and comparison of vitellogenin responses in rare minnow and zebrafish (Danio rerio)

The Science of the Total Environment
Tao Liao Ying Xu

Abstract

A competitive enzyme-linked immunosorbent assay (ELISA) was developed to determine vitellogenin (Vtg) in rare minnow (Gobiocypris rarus) based on the separation and purification of rare minnow Vtg (r-Vtg) as well as the production of polyclonal antibody against r-Vtg in rabbits. Three different ELISAs for measuring r-Vtg were then compared: (1) indirect ELISA with the antibody against carp (Cyprinus carpio) Vtg (c-Vtg) (IC-ELISA); (2) competitive ELISA with the antibody against c-Vtg, and using r-Vtg for coating the plates and preparing standard curve (CC-ELISA); (3) competitive ELISA with the antibody against r-Vtg, and using r-Vtg for coating the plates and preparing standard curve (CR-ELISA). The result showed that the homologous CR-ELISA was the most sensitive among the three assays for quantifying r-Vtg. The sensitivities to 17alpha-ethinylestradiol (EE(2)) of rare minnow and zebrafish (Danio rerio) were compared upon the establishment of homologous competitive ELISA. The lowest observed effect concentrations (LOECs) to induce Vtg were found to be 0.8 ng EE(2) l(-1) for rare minnow and 4 ng EE(2) l(-1) for zebrafish respectively. Afterwards, CR-ELISA was applied to measure Vtg concentration in whole body homogenate (WBH) o...Continue Reading

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Citations

Nov 13, 2013·Fish Physiology and Biochemistry·Xiaozheng ZhangSiyu Mao
Apr 30, 2013·Aquatic Toxicology·Katalin BakosBalázs Kovács
Feb 5, 2011·Diseases of Aquatic Organisms·Yu RaoHeping Dai
Sep 13, 2014·Environmental Toxicology·Xiaoxi YangGuibin Jiang
Jul 27, 2011·Environmental Toxicology and Pharmacology·Tao LiaoYing Xu
Jul 21, 2009·The Journal of Steroid Biochemistry and Molecular Biology·M M EsterhuyseJ H van Wyk
Dec 1, 2007·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Yanhong WeiJiayin Dai
Mar 17, 2016·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Jun WangShaoguo Ru
Sep 11, 2007·Environmental Pollution·Thomas R RainwaterScott T McMurry
Mar 5, 2008·Analytical and Bioanalytical Chemistry·Maoyong SongGuibin Jiang

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