PMID: 2109016Apr 17, 1990Paper

An enzyme-linked immunosorbent assay for the determination of human IgG subclass antibodies directed against Branhamella catarrhalis

Journal of Immunological Methods
D GoldblattM W Turner

Abstract

An ELISA procedure to determine the distribution of human IgG subclass antibodies directed against the gram-negative bacterium Branhamella catarrhalis has been developed using commercially available monoclonal anti-IgG subclass antibodies. Using whole bacteria as coating antigen the specificity of the assay was determined and showed minimal cross-reactivity with a range of other bacteria. Estimations of IgG1, IgG2, IgG3, IgG4 and total IgG antibodies directed against this antigen were performed. All normal adult sera tested had measurable antibody levels of specific IgG1, IgG2, IgG3 and total IgG. Specific IgG4 was undetectable in the majority of adult sera. These assays will be of value for investigation of both children and adults with suspected immunodeficiency and recurrent upper respiratory tract infection.

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Citations

Oct 1, 1992·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·I JönssonM Thorén
Apr 1, 1993·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·I JönssonM Thorén
Apr 1, 1995·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M RahmanA Krook
Dec 3, 1993·Journal of Immunological Methods·D GoldblattM W Turner
Nov 26, 1999·The Pediatric Infectious Disease Journal·K MathersD Goldblatt
Mar 1, 1997·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·M RahmanA Krook
Jan 10, 2002·Clinical Microbiology Reviews·Cees M VerduinAlex van Belkum
Nov 22, 2005·Infection and Immunity·Nadja HeinigerChristoph Aebi
Nov 14, 1997·Infection and Immunity·C AebiE J Hansen

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