An enzymic analysis of NADPH production and consumption in Candida utilis

Journal of General Microbiology
P M BruinenbergW A Scheffers

Abstract

Candida utilis CBS 621 was grown in chemostat cultures at D = 0.1 h-1 on glucose, xylose, gluconate, acetate, or ethanol as the growth-limiting substrate with ammonia or nitrate as the nitrogen source and analysed for NADPH-producing and NADPH-consuming enzyme activities. Nitrate and nitrite reductases were strictly NADPH-dependent. For all carbon sources, growth with nitrate resulted in elevated levels of HMP pathway enzymes. NADP+-linked isocitrate dehydrogenase did not vary significantly with the NADPH requirement for biosynthesis. Growth on ethanol strongly enhanced activity of NADP+-linked aldehyde dehydrogenase. Neither NADP+-linked malic enzyme nor transhydrogenase activities were detectable under any of the growth conditions. The absence of transhydrogenase was confirmed by the enzyme profiles of cells grown on mixtures of glucose and formate. It is concluded that the HMP pathway and possibly NADP+-linked isocitrate dehydrogenase are the major sources of NADPH in Candida utilis.

Citations

Apr 1, 1986·Molecular & General Genetics : MGG·C Kohchi, A Toh-e
Apr 1, 1997·Applied Biochemistry and Biotechnology·V W Yang, T W Jeffries
Jan 12, 2007·Applied Microbiology and Biotechnology·Tom Birger GranströmMatti Leisola
Nov 28, 2012·World Journal of Microbiology & Biotechnology·Changying Guo, Ning Jiang
Sep 9, 2000·FEMS Microbiology Reviews·C L FloresC Gancedo
Oct 20, 2005·Journal of Bioscience and Bioengineering·Gianni Panagiotou, Paul Christakopoulos
Dec 22, 2009·Applied and Environmental Microbiology·Jin HouGoutham N Vemuri
Feb 5, 2008·Applied and Environmental Microbiology·César FonsecaIsabel Spencer-Martins
Feb 17, 2009·Applied and Environmental Microbiology·Dawid BratBeate Wiedemann
Nov 6, 2012·Applied and Environmental Microbiology·Suriana SabriClaudia E Vickers
Dec 8, 2005·Applied and Environmental Microbiology·Anneli NilssonGunnar Lidén
Aug 8, 2008·Microbial Cell Factories·Beate Pscheidt, Anton Glieder
May 26, 2012·PloS One·Yasuyuki TomitaShigehito Ikushima
Sep 3, 2010·Applied Microbiology and Biotechnology·Zheng ZhaoPeter J T Verheijen
Jul 19, 1996·Molecular & General Genetics : MGG·J P van den HomberghJ Visser
Jan 1, 1986·Antonie van Leeuwenhoek·P M Bruinenberg
Oct 1, 1991·Antonie van Leeuwenhoek·C Verduyn
Jan 1, 1991·Antonie van Leeuwenhoek·C VerduynJ P van Dijken
Jun 5, 2007·Applied and Environmental Microbiology·Eva AlbersLena Gustafsson
Jan 15, 1999·Critical Reviews in Biotechnology·P Chandrakant, V S Bisaria
Jan 1, 1989·Critical Reviews in Biotechnology·H Schneider
Apr 20, 1995·Biotechnology and Bioengineering·H JørgensenH Møllgaard
Oct 15, 2013·Biotechnology Progress·Songxin YangJian Chen
Jul 30, 2015·International Journal of Food Microbiology·Valentin TilloySylvie Dequin
Jun 27, 2006·Metabolic Engineering·Gianni PanagiotouLisbeth Olsson
Jun 26, 2015·FEMS Yeast Research·W Alexander Scheffers

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.