PMID: 15219152Jun 29, 2004Paper

An EP overexpression screen for genetic modifiers of Notch pathway function in Drosophila melanogaster

Genetical Research
Lauren E HallB Yedvobnick

Abstract

The Notch pathway comprises a signal transduction cascade required for the proper formation of multiple tissues during metazoan development. Originally described in Drosophila for its role in nervous system formation, the pathway has attracted much wider interest owing to its fundamental roles in a range of developmental and disease-related processes. Despite extensive analysis, Notch signaling is not completely understood and it appears that additional components of the pathway remain to be identified and characterized. Here, we describe a novel genetic strategy to screen for additional Notch pathway genes. The strategy combines partial loss of function for pathway activity with Enhancer-promoter (EP)-induced overexpression of random loci across the dorsoventral wing margin. Mastermind (Mam) is a nuclear component of the Notch signaling cascade. Using a GAL4-UAS-driven dominant-negative form of Mam, we created a genotype that exhibits a completely penetrant dominant wing-nicking phenotype. This phenotype was assayed for enhancement or suppression after outcrossing to several thousand EP lines. The screen identified known components or modifiers of Notch pathway function, as well as several potential new components. Our results...Continue Reading

Citations

Sep 26, 2013·The Journal of Experimental Medicine·Marie Le BouteillerMichel Cohen-Tannoudji
Oct 21, 2010·BMC Developmental Biology·Philippos MourikisBrian S DeDecker
Jul 9, 2010·The FEBS Journal·Giuseppe TortorielloMaria Furia
May 27, 2014·BioMed Research International·Julia M I Barth, Katja Köhler
May 23, 2006·Mechanisms of Development·Stefanie AlbrechtAchim Paululat
Oct 17, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Wooly PierreBarry Yedvobnick
May 13, 2018·G3 : Genes - Genomes - Genetics·Kaitlyn EllisBarry Yedvobnick
Jan 15, 2008·Development·Penelope HaywardAlfonso Martinez Arias

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