An epidemiological comparative study on diagnosis of rodent leptospirosis in Mazandaran Province, northern Iran

Epidemiology and Health
Behzad EsfandiariHossein Nahrevanian

Abstract

Leptospirosis is a zoonosis caused by leptospires, in which transmission occurs through contact with contaminated biological fluids from infected animals. Rodents can act as a source of infection for humans and animals. The disease has a global distribution, mainly in humid, tropical and sub-tropical regions. The aim of this study was to compare culture assays, the microscopic agglutination test (MAT), polymerase chain reaction (PCR), and nested PCR (n-PCR), for the diagnosis of leptospirosis in rodents in Mazandaran Province, northern Iran. One hundred fifty-one rodents were trapped alive at 10 locations, and their urine and kidney samples were collected and used for the isolation of live Leptospira. The infecting serovars were identified and the antibody titres were measured by MAT, using a panel of 20 strains of live Leptospira species as antigens. The presence of leptospiral DNA was evaluated in urine and kidney samples using PCR and n-PCR. No live leptospires were isolated from the kidney and urine samples of the rodents. Different detection rates of leptospirosis were observed with MAT (21.2%), PCR (11.3%), and n-PCR (3.3%). The dominant strain was Leptospira serjoehardjo (34.4%, p=0.28), although other serotypes were als...Continue Reading

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Citations

Aug 10, 2019·PLoS Neglected Tropical Diseases·Kenneth BoeySreekumari Rajeev
Apr 20, 2018·PLoS Neglected Tropical Diseases·Mohammad Hasan RabieeEhsan Mostafavi
Sep 23, 2019·Acta Tropica·Zoey May Pheng SooRuqaiyyah Siddiqui
Mar 28, 2020·Parasite Epidemiology and Control·Azar ShokriSeyed Ahmad Hashemi

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Methods Mentioned

BETA
PCR
electrophoresis
dot-blot

Software Mentioned

SPSS

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