PMID: 9435226Jan 22, 1998Paper

An ERp60-like protein from the filarial parasite Dirofilaria immitis has both transglutaminase and protein disulfide isomerase activity

Proceedings of the National Academy of Sciences of the United States of America
R ChandrashekarK Mehta

Abstract

Transglutaminases (TGases; EC 2.3.2.13) are a family of enzymes that catalyze calcium-dependent covalent cross-linking of cellular proteins by establishing epsilon-(gamma-glutamyl)lysine isopeptide bonds. These covalent isopeptide bonds are of great physiological significance because they are highly resistant to proteolysis, denaturants, and reducing agents. Prior studies have demonstrated the presence of isopeptide bonds in the sheath and cuticle of filarial parasites, suggesting an important role for TGase-catalyzed reactions during the growth and development of filarial nematodes. Herein we report the identification and cloning of a cDNA encoding a TGase from the dog heartworm Dirofilaria immitis (DiTG). The DiTG expressed in Escherichia coli (recombinant DiTG) was able to catalyze calcium-dependent cross-linking reactions. The derived amino acid sequence of the DiTG cDNA (pDiTG) predicts a protein of 57.1 kDa and includes an N-terminal hydrophobic signal peptide. The pDiTG has no sequence similarity with any of the known TGases, but it has significant homology to protein disulfide isomerase (PDI) and, particularly, to the PDI-related endoplasmic reticulum protein ERp60, a PDI isoform found in the lumen of endoplasmic reticu...Continue Reading

References

Jan 2, 1992·Nature·M J Gething, J Sambrook
Dec 31, 1990·Biochemical and Biophysical Research Communications·K MehtaP J Birckbichler
Jan 1, 1985·Annual Review of Biochemistry·A Holmgren
Aug 1, 1994·Trends in Biochemical Sciences·R B FreedmanM F Tuite
Oct 15, 1994·European Journal of Biochemistry·R N Singh, K Mehta
Nov 1, 1993·Experimental Parasitology·R M MaizelsM E Selkirk
Apr 15, 1993·European Journal of Biochemistry·P N VanH D Söling
Sep 1, 1995·Antimicrobial Agents and Chemotherapy·S LustigmanA M Prince
Dec 1, 1995·The International Journal of Biochemistry & Cell Biology·R N SinghK Mehta
Oct 1, 1996·International Journal for Parasitology·M Blaxter, L Liu

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Citations

Jun 17, 2005·Analytical Biochemistry·Zoltan NemesLászló Fésüs
Mar 20, 2004·Pharmacology & Therapeutics·Amere Subbarao Sreedhar, Peter Csermely
Mar 14, 2003·International Journal for Parasitology·P GeldhofE Claerebout
Mar 8, 2000·International Journal for Parasitology·N TsujiS Yoshihara
Mar 5, 2003·Molecular and Biochemical Parasitology·Isabel VercauterenJozef Vercruysse
Jan 8, 1999·Molecular and Biochemical Parasitology·N TsujiR Chandrashekar
Sep 11, 1999·The International Journal of Biochemistry & Cell Biology·J S Chen, K Mehta
Jan 22, 2010·The Journal of Biological Chemistry·Florentina PenaIneke Braakman
Jul 18, 2008·Cancer Research·Jee Young HwangAnil K Sood
May 15, 2001·Biochemical and Biophysical Research Communications·A MádiL Fésüs
Aug 7, 2002·Bioscience, Biotechnology, and Biochemistry·Hirokazu OkudoReiko Urade
May 5, 1999·Bioscience, Biotechnology, and Biochemistry·R UradeM Kito
May 9, 2007·Infection and Immunity·Naotoshi TsujiKozo Fujisaki
Oct 14, 2004·Molecular and Biochemical Parasitology·B J DavidsF D Gillin
Feb 27, 2004·Biochemical and Biophysical Research Communications·András MádiLászló Fésüs
Apr 10, 2003·Biochemical and Biophysical Research Communications·Bernadett BlaskóLászló Fésüs
Oct 22, 2011·Biochimica Et Biophysica Acta·Soner GundemirGail V W Johnson
Jul 19, 2001·The Journal of Veterinary Medical Science·H Kasug-AokiT Isobe
Dec 22, 1999·Seminars in Cell & Developmental Biology·R Noiva
Sep 19, 2006·Biochimica Et Biophysica Acta·Pier Giorgio MastroberardinoMauro Piacentini
Jan 24, 2006·Journal of Biochemistry·Hirokazu OkudoReiko Urade
Sep 6, 2000·The Journal of Biological Chemistry·L A KnodlerF D Gillin
Nov 2, 2019·Critical Reviews in Food Science and Nutrition·Yi Zhang, Benjamin K Simpson
Oct 9, 1999·The Journal of Biological Chemistry·L A KnodlerF D Gillin
Apr 23, 2017·Immunologic Research·Christiana ImmanuelKaliraj Perumal
Dec 28, 2002·The Journal of Biological Chemistry·Myungjo YouKozo Fujisaki
Jul 5, 2006·Molecular Cancer Therapeutics·Jansina Y FokKapil Mehta
Feb 11, 2021·Pathogens·Anna Zawistowska-DeniziakDaniel Młocicki

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