An Escherichia coli Effector Protein EspF May Induce Host DNA Damage via Interaction With SMC1.

Frontiers in Microbiology
Muqing FuChengsong Wan

Abstract

Enterohemorrhagic Escherichia coli (EHEC) O157: H7 is an important foodborne pathogen that causes human diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome. EspF is one of the most important effector proteins injected by the Type III Secretion System. It can target mitochondria and nucleoli, stimulate host cells to produce ROS, and promote host cell apoptosis. However, the mechanism of the host-pathogen interaction leading to host oxidative stress and cell cytotoxic effects such as DNA damage remains to be elucidated. Here, we used Cell Counting Kit-8 (CCK-8) assays and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-OHdG) ELISA to study cell viability and DNA oxidative damage level after exposure to EspF. Western blot and immunofluorescence were also used to determine the level of the DNA damage target protein p-H2AX and cell morphology changes after EspF infection. Moreover, we verified the toxicity in intestinal epithelial cells mediated by EspF infection in vivo. In addition, we screened the host proteins that interact with EspF using CoIP-MS. We found that EspF may more depend on its C-terminus to interact with SMC1, and EspF could activate SMC1 phosphorylation and migrate it to the cytoplasm. In summary, this study reveal...Continue Reading

References

Aug 1, 1990·Infection and Immunity·E A WadolkowskiA D O'Brien
Sep 26, 2001·The Journal of Biological Chemistry·S BurmaD J Chen
Sep 1, 1962·The Journal of Infectious Diseases·M BOHNHOFF, C P MILLER
Feb 28, 2004·The Journal of Biological Chemistry·Philip R HardwidgeB Brett Finlay
Mar 23, 2005·Lancet·Phillip I TarrWayne L Chandler
Jul 28, 2006·Cold Spring Harbor Symposia on Quantitative Biology·R Kitagawa, M B Kastan
Aug 1, 2006·Experimental Cell Research·Erwan Watrin, Jan-Michael Peters
Dec 18, 2007·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Alexander R Ball, Kyoko Yokomori
Aug 4, 2010·Infection and Immunity·Ashleigh HolmesPaul Dean
Jan 11, 2011·Mutation Research·Thomas B KrystonAlexandros G Georgakilas
Jan 29, 2011·Journal of Biomedicine & Biotechnology·Krystle L Mohawk, Alison D O'Brien
Apr 15, 2011·Molecular Microbiology·Alexander R C WongElizabeth L Hartland
Aug 25, 2012·Frontiers in Cellular and Infection Microbiology·Alline R Pacheco, Vanessa Sperandio
Jan 11, 2014·Infectious Agents and Cancer·Peter C TyrerJacqueline I Keenan
Sep 1, 2017·Nucleic Acids Research·Chao LiuXiaochun Yu
Sep 20, 2017·International Journal of Biological Sciences·Fei YiXiaoyu Song
Sep 30, 2017·Cancer Treatment Reviews·Laura Carrassa, Giovanna Damia
Dec 5, 2017·Current Opinion in Immunology·Veronika Horn, Antigoni Triantafyllopoulou
Apr 21, 2018·Cellular and Molecular Gastroenterology and Hepatology·John J GarberScott B Snapper
Aug 9, 2018·Current Topics in Microbiology and Immunology·Sabrina L SlaterGad Frankel
Dec 14, 2018·Frontiers in Microbiology·Ying HuaChengsong Wan
Dec 19, 2018·Acta Bio-medica : Atenei Parmensis·Gian Luigi De' AngelisFrancesca Negri
Nov 24, 2019·Neoplasia : an International Journal for Oncology Research·Casey D StefanskiJenifer R Prosperi

❮ Previous
Next ❯

Software Mentioned

ImageJ
COG
SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis