An Escherichia coli strain, PGB01, isolated from feral pigeon Faeces, thermally fit to survive in pigeon, shows high level resistance to trimethoprim

PloS One
Arvind KumarRanadhir Chakraborty

Abstract

In this study, of the hundred Escherichia coli strains isolated from feral Pigeon faeces, eighty five strains were resistant to one or more antibiotics and fifteen sensitive to all the antibiotics tested. The only strain (among all antibiotic-resistant E. coli isolates) that possessed class 1 integron was PGB01. The dihydrofolate reductase gene of the said integron was cloned, sequenced and expressed in E. coli JM109. Since PGB01 was native to pigeon's gut, we have compared the growth of PGB01 at two different temperatures, 42°C (normal body temperature of pigeon) and 37°C (optimal growth temperature of E. coli; also the human body temperature), with E. coli K12. It was found that PGB01 grew better than the laboratory strain E. coli K12 at 37°C as well as at 42°C. In the thermal fitness assay, it was observed that the cells of PGB01 were better adapted to 42°C, resembling the average body temperature of pigeon. The strain PGB01 also sustained more microwave mediated thermal stress than E. coli K12 cells. The NMR spectra of the whole cells of PGB01 varied from E. coli K12 in several spectral peaks relating some metabolic adaptation to thermotolerance. On elevating the growth temperature from 37°C to 42°C, susceptibility to kanam...Continue Reading

References

May 1, 1971·Journal of General Microbiology·P H Ray, T D Brock
Jul 1, 1981·American Journal of Clinical Pathology·P A MackowiakJ P Luby
Jan 1, 1995·Antimicrobial Agents and Chemotherapy·C LévesqueP H Roy
Jul 1, 1994·The Journal of Infectious Diseases·P A Chapman, C A Siddons
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·A M LeroiR E Lenski
Jan 1, 1996·Scandinavian Journal of Infectious Diseases·E ReisingerG Krejs
Oct 12, 1999·Microbiology·T CaldasG Richarme
Nov 18, 2000·The Journal of Biological Chemistry·S R DowdJ L Patton-Vogt
Nov 18, 2000·Antimicrobial Agents and Chemotherapy·L P KotraS Mobashery
Jun 30, 2001·Evolution; International Journal of Organic Evolution·V S CooperR E Lenski
Oct 8, 2005·The Journal of Veterinary Medical Science·Chikako TanakaNaotaka Ishiguro
Feb 7, 2006·Journal of Molecular Graphics & Modelling·Junmei WangDavid A Case
Oct 3, 2006·Traffic·Naeko Shinozaki-NarikawaYoshikazu Shibasaki
Jun 8, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Alexander W FriedrichHelge Karch
Nov 22, 2007·Proceedings. Biological Sciences·Elizabeth A OstrowskiRichard E Lenski
Jul 7, 2010·Journal of Applied Microbiology·T RadimerskyI Literak
May 21, 2011·Annals of Clinical Microbiology and Antimicrobials·Arvind KumarRanadhir Chakraborty
May 29, 2012·Applied and Environmental Microbiology·Elizabeth Ponce-RivasAshraf A Khan

❮ Previous
Next ❯

Citations

Jun 11, 2021·Frontiers in Veterinary Science·Dorota Chrobak-ChmielMagdalena Rzewuska

❮ Previous
Next ❯

Datasets Mentioned

BETA
HM486679
FN563072

Methods Mentioned

BETA
PCR
Nuclear magnetic resonance
NMR

Software Mentioned

AMBER11
CLUSTAL W
Galaxy Refine
GalaxyTBM
ERRAT
SAVES
Autodock
BlastN

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.