PMID: 9164483May 1, 1997Paper

An Escherichia coli topB mutant increases deletion and frameshift mutations in the supF target gene

Mutation Research
N UematsuK Yamamoto

Abstract

We have improved a system to examine forward mutations that occurred in the supF gene of Escherichia coli placed on a multicopy plasmid. Using this system, we determined the mutational specificity for a topB deletion mutator in which topoisomerase III is hampered. The frequency of supF- mutations in topB strain was 4.9 x 10(-7), that is essentially the same as that in wild-type strain, 3.1 x 10(-7). Half the number of the supF- mutations were large deletions, where a specific deletion among a 10-base pair direct repeat dominated, but other types of deletions were also found. Most of the deletions were associated with the presence of directly repeated sequences capable of accounting for their endpoints. Frameshift mutations in topB strain also significantly increased compared with those of wild-type (17 vs. 2%). Base substitutions comprised 27% of the events, specificity of which in topB strain was the same as that in wild-type strain. The present data suggest that topB is a novel class of mutator that strongly induces repeated sequence dependent deletion mutagenesis and high frequencies of frameshift mutagenesis.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Nov 1, 1989·Molecular & General Genetics : MGG·H IkehataT Kato
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H Ikeda
Jul 20, 1988·Journal of Molecular Biology·B S Singer, J Westlye
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H IkedaA Naito
Jan 1, 1982·Molecular & General Genetics : MGG·I M JonesS D Ehrlich
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·A M AlbertiniJ H Miller
Jun 3, 1994·Molecular & General Genetics : MGG·S Akasaka, K Yamamoto

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Citations

Jun 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tina Wilson-Sali, Tao-Shih Hsieh
Sep 17, 2005·Molecular Microbiology·Christopher R LopezE Lynn Zechiedrich
Oct 17, 2012·Molecular Microbiology·Brenda A Perez-CheeksKenneth J Marians
Mar 14, 2000·The Journal of Biological Chemistry·E L ZechiedrichN R Cozzarelli
Mar 20, 2021·Mutation Research·Marc BicharaIain B Lambert
Aug 17, 2021·Protein Science : a Publication of the Protein Society·Alexandre Duprey, Eduardo A Groisman

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