An essential amino acid residue in the protein translocation channel revealed by targeted random mutagenesis of SecY

Proceedings of the National Academy of Sciences of the United States of America
H Mori, K Ito

Abstract

The SecY/Sec61alpha family of membrane proteins are the central subunits of the putative protein translocation channel. We introduced random mutations into a segment of Escherichia coli SecY within its cytoplasmic domain 5, which was shown previously to be important for the SecA-dependent translocation activity. Mutations were classified into those retaining function and those gaining a dominant-interfering ability caused by a loss of function. These analyses showed that Arg-357, Pro-358, Gly-359, and Thr-362 are functionally important; Arg-357, conserved in almost all organisms, was identified as an indispensable residue.

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Citations

Apr 12, 2011·Cellular and Molecular Life Sciences : CMLS·Ilja Kusters, Arnold J M Driessen
Oct 13, 2001·Trends in Microbiology·H Mori, K Ito
Jun 26, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Nobuyuki ShimohataYoshinori Akiyama
Mar 2, 2012·Proceedings of the National Academy of Sciences of the United States of America·Kush DalalFranck Duong
Jan 24, 2012·The Journal of Biological Chemistry·Zht Cheng WuArnold J M Driessen
May 7, 2013·The Journal of Biological Chemistry·Denis G KnyazevPeter Pohl
Nov 6, 2010·Plant Physiology·Courtney A SkalitzkyDonna E Fernandez
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Dec 18, 2012·Biochemistry. Biokhimii︠a︡·T F Solov'evaO Yu Portnyagina
Dec 15, 2007·Annual Review of Biochemistry·Arnold J M Driessen, Nico Nouwen
Oct 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hiroyuki Mori, Koreaki Ito
Aug 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Akiko MurakamiKoreaki Ito
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