PMID: 11345435May 10, 2001Paper

An essential protein-binding domain of nuclear RNase P RNA

RNA
W A ZiehlerD R Engelke

Abstract

Eukaryotic RNase P and RNase MRP are endoribonucleases composed of RNA and protein subunits. The RNA subunits of each enzyme share substantial secondary structural features, and most of the protein subunits are shared between the two. One of the conserved RNA subdomains, designated P3, has previously been shown to be required for nucleolar localization. Phylogenetic sequence analysis suggests that the P3 domain interacts with one of the proteins common to RNase P and RNase MRP, a conclusion strengthened by an earlier observation that the essential domain can be interchanged between the two enzymes. To examine possible functions of the P3 domain, four conserved nucleotides in the P3 domain of Saccharomyces cerevisiae RNase P RNA (RPR1) were randomized to create a library of all possible sequence combinations at those positions. Selection of functional genes in vivo identified permissible variations, and viable clones that caused yeast to exhibit conditional growth phenotypes were tested for defects in RNase P RNA and tRNA biosynthesis. Under nonpermissive conditions, the mutants had reduced maturation of the RPR1 RNA precursor, an expected phenotype in cases where RNase P holoenzyme assembly is defective. This loss of RPR1 RNA m...Continue Reading

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Citations

Mar 10, 2001·Journal of Cellular Physiology·S XiaoD R Engelke
Jun 11, 2002·Methods : a Companion to Methods in Enzymology·David S BernsteinMarvin Wickens
Oct 31, 2002·RNA·Chatchawan SrisawatDavid R Engelke
Jul 16, 2004·Nucleic Acids Research·Xing LiLasse Lindahl
Aug 10, 2005·Nucleic Acids Research·Paul PiccinelliTore Samuelsson
Apr 1, 2011·Nucleic Acids Research·Robert ReinerNayef Jarrous
May 30, 2012·Nucleic Acids Research·Mario KrehanAstrid Schön
Jan 9, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Anna PerederinaAndrey S Krasilnikov
Jun 5, 2002·Annual Review of Biochemistry·Shaohua XiaoDavid R Engelke
Mar 26, 2010·BMC Bioinformatics·Feng-Jie Sun, Gustavo Caetano-Anollés
Jul 16, 2010·RNA·Olga Esakova, Andrey S Krasilnikov
Mar 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Felicia Houser-ScottDavid R Engelke
May 10, 2013·Annual Review of Biophysics·Alfonso Mondragón
Apr 6, 2006·Critical Reviews in Biochemistry and Molecular Biology·Scott C Walker, David R Engelke
Jul 31, 2012·Methods : a Companion to Methods in Enzymology·Franck Martin
Jul 28, 2004·Journal of Molecular Biology·Scott C Walker, Johanna M Avis
Sep 17, 2013·Wiley Interdisciplinary Reviews. RNA·Dominika WichtowskaMagdalena Boguta
Jan 16, 2010·The EMBO Journal·Anna PerederinaAndrey S Krasilnikov
May 9, 2006·Trends in Biochemical Sciences·Donald EvansNorman R Pace
Nov 4, 2006·Molecular Cell·Steven M MarquezNorman R Pace
Jan 7, 2004·FEBS Letters·Sophie JaegerFranck Martin
Jun 5, 2010·RNA Biology·Anna Perederina, Andrey S Krasilnikov
Feb 12, 2021·Journal of Structural Biology·Changping YinJing Huang

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