An essential role for hfq involved in biofilm formation and virulence in serotype 4b Listeria monocytogenes

Microbiological Research
Hao YaoYuelan Yin

Abstract

Regulator factor Hfq has been widely detected among both Gram-positive and Gram-negative bacteria; however, its role in Gram-positive bacteria is less well established and varies among species. In Listeria monocytogenes (Lm), an organism able to adapt to a range of environments and live both saprobiotic and parasitic lifestyles, the role of Hfq is not fully understood. Serotype 4b Listeria monocytogenes strains associated with the majority of listeriosis outbreak, while the function of hfq in serotype 4b strains still not referenced. Here, we constructed hfq deletion and reversion mutants of serotype 4b Lm NTSN and analysed the biological characteristics both in vitro and in vivo. The deletion of hfq resulted in a growth deficiency in medium containing 4.5% ethanol or 1% Triton X-100, and the growth of the mutant was significantly reduced at 4 °C. Furthermore, the hfq deletion dramatically decreased biofilm formation in BHI medium and gastric fluid medium, and reduced the invasion and replication rate into the Caco-2BBe cells and RAW264.7 cells. However, complementation restored the wild-type phenotype. Importantly, mouse infection experiments demonstrated that hfq played a more important role in the colonisation and virulence ...Continue Reading

Citations

Dec 29, 2020·Frontiers in Microbiology·Fanzeng MengYuelan Yin
Nov 5, 2020·International Journal of Peptide Research and Therapeutics·Shivani SrivastavaAjay Kumar
Mar 3, 2021·Food Research International·Tassiana RamiresWladimir Padilha da Silva
Aug 28, 2021·International Journal of Molecular Sciences·Lidia GaffkeGrzegorz Węgrzyn

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.