An essential role of sialylated O-linked sugar chains in the recognition of mouse CD99 by paired Ig-like type 2 receptor (PILR).

The Journal of Immunology : Official Journal of the American Association of Immunologists
Jing WangHisash Arase

Abstract

The paired Ig-like type 2 receptor (PILR), which comprises both inhibitory and activating isoforms, is well conserved among most mammalians. The inhibitory PILRalpha possesses an ITIM in its cytoplasmic domain, whereas the activating PILRbeta does not have an ITIM but transduces activating signals by associating with the ITAM-bearing DAP12 adapter molecule. Both mouse PILRalpha and PILRbeta recognize mouse CD99, which is broadly expressed on various cells, including lymphocytes, and is involved in the regulation of immune responses. We herein report that sialylated O-linked sugar chains on CD99 are essential for the recognition by PILR. Mutations of one of two O-glycosylation sites on CD99 significantly reduced recognition of CD99 by the activating PILRbeta, whereas recognition by the inhibitory PILRalpha was not affected. In contrast, mutations of both O-glycosylation sites on CD99 completely abrogated the recognition by both PILRalpha and PILRbeta. PILR did not recognize CD99 treated with neuraminidase, and CD99 expressed on cells transfected with core 2 beta-1,6-N-acetylglucosaminyltransferase was not recognized by PILR. NK cells expressing endogenous activating PILRbeta receptors mediated cytotoxicity against cells expressi...Continue Reading

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Citations

Feb 1, 2008·The Journal of Biological Chemistry·Shigekazu TabataKatsumi Maenaka
May 21, 2014·Proceedings of the National Academy of Sciences of the United States of America·Qiong LuGeorge F Gao
Jun 4, 2014·Proceedings of the National Academy of Sciences of the United States of America·Kimiko KurokiKatsumi Maenaka
Jun 23, 2010·Immunology and Cell Biology·Hollie J PegramMichael H Kershaw
Feb 25, 2015·International Immunology·Kazuki KishidaHisashi Arase
Sep 25, 2014·PloS One·Jorge Luis Ayala-LujanFernando Ruiz-Perez
Sep 12, 2020·Current Drug Targets·Adekunle Babajide RowaiyeCharles Okechukwu Esimone

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