PMID: 9190206Feb 1, 1997Paper

An essential role of the yeast pheromone-induced Ca2+ signal is to activate calcineurin

Molecular Biology of the Cell
J L WitheeMartha S Cyert

Abstract

Previous studies showed that, in wild-type (MATa) cells, alpha-factor causes an essential rise in cytosolic Ca2+. We show that calcineurin, the Ca2+/calmodulin-dependent protein phosphatase, is one target of this Ca2+ signal. Calcineurin mutants lose viability when incubated with mating pheromone, and overproduction of constitutively active (Ca(2+)-independent) calcineurin improves the viability of wild-type cells exposed to pheromone in Ca(2+)-deficient medium. Thus, one essential consequence of the pheromone-induced rise in cytosolic Ca2+ is activation of calcineurin. Although calcineurin inhibits intracellular Ca2+ sequestration in yeast cells, neither increased extracellular Ca2+ nor defects in vacuolar Ca2+ transport bypasses the requirement for calcineurin during the pheromone response. These observations suggest that the essential function of calcineurin in the pheromone response may be distinct from its modulation of intracellular Ca2+ levels. Mutants that do not undergo pheromone-induced cell cycle arrest (fus3, far1) show decreased dependence on calcineurin during treatment with pheromone. Thus, calcineurin is essential in yeast cells during prolonged exposure to pheromone and especially under conditions of pheromone-...Continue Reading

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Citations

Nov 19, 2003·Biochemical and Biophysical Research Communications·Martha S Cyert
Aug 6, 2002·Cell Calcium·L J LombardíaM E Cerdán
Mar 6, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·A Omolola Eniola, Daniel A Hammer
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