An EST-SSR marker tightly linked to the barley male sterility gene (msg6) located on chromosome 6H

The Journal of Heredity
Livinus C Emebiri

Abstract

The barley male sterility gene (msg6) located on chromosome 6H has been used in breeding and research since its discovery 7 decades ago, but to date, no research has been reported that linked the gene with molecular markers. The main objective of this study was to identify expressed sequence tag-simple sequence repeat (EST-SSR) markers linked to msg6 as this could provide opportunities for gene discovery. In a cross of a male sterile line (04-042B) with a fully fertile line (VB0330; VB9524/Mundah), male sterility segregated in a 3:1 ratio of fertile to completely sterile plants (χ(2) = 0.03, P(0.05) = 0.95), in a population of 250 F(2) plants. Multipoint linkage mapping placed the msg6 gene at 4.9 cM from the EST-SSR, GBM1267, whereas 2-point analysis estimated a recombination fraction of 0.05 ± 0.02 (logarithm of the odds score = 26.34) between the EST-SSR and the male sterility gene. Multiple interval quantitative trait locus (QTL) analysis of spike weight, an indicative measure of reproductive success, identified a QTL near GBM1267 as having a major influence, explaining 68.7% of the variation in weight of individual spikes. The GBM1267 marker segregated in a 1:2:1 ratio, which makes it highly desirable for marker-assisted s...Continue Reading

References

Feb 18, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·T ThielA Graner
Nov 11, 2003·Trends in Plant Science·Jeppe R Andersen, Thomas Lübberstedt
Oct 23, 2004·Genome Génome / Conseil National De Recherches Canada·Ju-Kyung YuMark E Sorrells
Feb 21, 2006·Journal of Experimental Botany·Hisatomi Harada, Roger A Leigh
Jan 11, 2007·Genome Génome / Conseil National De Recherches Canada·Marion S RöderWinfriede Weschke
Mar 9, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·R K VarshneyA Graner
Sep 22, 2009·Genome Génome / Conseil National De Recherches Canada·Livinus C Emebiri

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