PMID: 154537Jan 1, 1979

An estimate of the minimal frequency of cytolytic T lymphocyte effector cells generated in allogeneic reactions

Journal of Immunological Methods
H D Engers, F W Fitch

Abstract

A 'micro' modification of the standard 51Cr release assay was developed. With this assay, significant cytolysis was obtained employing 100--200 51Cr-labeled target cells and fewer than 100 lymphocytes obtained from mixed leukocyte cultures. A minimal estimate as to the actual frequency of effector cells was determined for both primary (1 cell in 30) and secondary (1 cells in 8) cytolytic T lymphocyte populations.

References

Jan 1, 1981·Journal of Immunological Methods·M A Skinner, J Marbrook
Nov 12, 1982·Journal of Immunological Methods·T Ezaki, J Marbrook
Apr 27, 1984·Journal of Immunological Methods·J Barrington-Leigh
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M SarmientoF W Fitch
Mar 1, 1981·The Journal of Experimental Medicine·D P DialynasF W Fitch
Jan 1, 1981·Immunological Reviews·S Gillis, J Watson

Citations

Aug 1, 1978·European Journal of Immunology·D CollavoM Nabholz

Related Concepts

Pathologic Cytolysis
T-Lymphocyte
Lymphocyte Culture Test, Mixed
Dose-Response Relationship, Immunologic
Leukocytes
Target Cell Count
Assay
Microbiology Procedure
Microcytotoxicity Test
Leptocyte

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