An ethanolic extract of Artemisia scoparia inhibits lipolysis in vivo and has antilipolytic effects on murine adipocytes in vitro

American Journal of Physiology. Endocrinology and Metabolism
Anik BoudreauJacqueline M Stephens

Abstract

An ethanolic extract of Artemisia scoparia (SCO) has metabolically favorable effects on adipocyte development and function in vitro and in vivo. In diet-induced obese mice, SCO supplementation significantly reduced fasting glucose and insulin levels. Given the importance of adipocyte lipolysis in metabolic health, we hypothesized that SCO modulates lipolysis in vitro and in vivo. Free fatty acids and glycerol were measured in the sera of mice fed a high-fat diet with or without SCO supplementation. In cultured 3T3-L1 adipocytes, the effects of SCO on lipolysis were assessed by measuring glycerol and free fatty acid release. Microarray analysis, qPCR, and immunoblotting were used to assess gene expression and protein abundance. We found that SCO supplementation of a high-fat diet in mice substantially reduces circulating glycerol and free fatty acid levels, and we observed a cell-autonomous effect of SCO to significantly attenuate tumor necrosis factor-α (TNFα)-induced lipolysis in cultured adipocytes. Although several prolipolytic and antilipolytic genes were identified by microarray analysis of subcutaneous and visceral adipose tissue from SCO-fed mice, regulation of these genes did not consistently correlate with SCO's abilit...Continue Reading

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Citations

Mar 25, 2019·Frontiers in Nutrition·Anik BoudreauJacqueline M Stephens
Feb 27, 2021·Journal of Ethnopharmacology·Jiwei DingHua Huang
Apr 9, 2021·Journal of Natural Products·David RibnickyGuido F Pauli
Jul 7, 2021·Obesity·Innocence Harvey, Jacqueline M Stephens

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Methods Mentioned

BETA
PCR
protein assay
electrophoresis
nuclear
nuclear translocation

Software Mentioned

Image Studio
CyberT
Limma
GraphPad Prism
SDS
Spectromax

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