An evaluation of a recombinant multiepitope based antigen for detection of Toxoplasma gondii specific antibodies

BMC Infectious Diseases
Khalid HajissaZeehaida Mohamed

Abstract

The inefficiency of the current tachyzoite antigen-based serological assays for the serodiagnosis of Toxoplasma gondii infection mandates the need for acquirement of reliable and standard diagnostic reagents. Recently, epitope-based antigens have emerged as an alternative diagnostic marker for the achievement of highly sensitive and specific capture antigens. In this study, the diagnostic utility of a recombinant multiepitope antigen (USM.TOXO1) for the serodiagnosis of human toxoplasmosis was evaluated. An indirect enzyme-linked immunosorbent assay (ELISA) was developed to evaluate the usefulness of USM.TOXO1 antigen for the detection of IgG antibodies against Toxoplasma gondii in human sera. Whereas the reactivity of the developed antigen against IgM antibody was evaluated by western blot and Dot enzyme immunoassay (dot-EIA) analysis. The diagnostic performance of the new antigens in IgG ELISA was achieved at the maximum values of 85.43% and 81.25% for diagnostic sensitivity and specificity respectively. The USM.TOXO1 was also proven to be reactive with anti- T. gondii IgM antibody. This finding makes the USM.TOXO1 antigen an attractive candidate for improving the toxoplasmosis serodiagnosis and demonstrates that multiepitope...Continue Reading

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Citations

Aug 21, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Amjad Hayat Khan, Rahmah Noordin
Feb 8, 2020·Parasite : Journal De La Société Française De Parasitologie·Loïc SimonUNKNOWN Toxoplasma p35 Study Group
May 19, 2020·Pathogens and Global Health·Mamdouh M HegazyNairmen Nabih
May 28, 2020·Frontiers in Cellular and Infection Microbiology·Rochelle Haidee D YbañezYoshifumi Nishikawa
Jul 9, 2020·International Journal of Environmental Research and Public Health·Mohammed Nasiru WanaRoslaini Abd Majid
Oct 13, 2019·Microbial Pathogenesis·Amirreza Javadi MamaghaniSeyyed Javad Seyyed Tabaei

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Methods Mentioned

BETA
PCR
ELISA

Software Mentioned

PS
MedCalc

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