Nov 1, 1975

An evaluation of factors affecting the in vitro bioassay for erythropoietin

Experimental Hematology
C D Dunn, J A Napier

Abstract

Two main aspects of the in vitro mouse foetal liver cell assay for Erythroid Stimulating Factor (ESF) in human sera have been investigated. The haem extraction process has been shown to give specific and quantitative recovery of 59Fe labelled haem from haemoglobin thus confirming that the material assayed in human sera is stimulating the synthesis of this protein. The extraction procedure can be simplified considerably by prior mixing of the reagents without significantly influencing the results. Several serum constituents (citrate, testosterone, B12, folic acid and iron) have been investigated over a range of concentrations for possible effects on the cultures. Generally only small effects on haem synthesis were observed. It is concluded that variations in the levels of these factors in sera from treated patients will not produce any significant alterations in the estimated ESF concentrations.

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Mentioned in this Paper

Testosterone Measurement
Fetal Structures
Epoetin Alfa
Epo
Erythropoietin Measurement
Citrate Measurement
Phosphoglycerate Dehydrogenase Activity
Hemoglobin Measurement
Eryhem
Erythropoietin

About this Paper

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