An evaluation of putative sympatric speciation within Limnanthes (Limnanthaceae).

PloS One
Stephen C MeyersRobert Meinke

Abstract

Limnanthes floccosa ssp. floccosa and L. floccosa ssp. grandiflora are two of five subspecies within Limnanthes floccosa endemic to vernal pools in southern Oregon and northern California. Three seasons of monitoring natural populations have quantified that L. floccosa ssp. grandiflora is always found growing sympatrically with L. floccosa ssp. floccosa and that their flowering times overlap considerably. Despite their subspecific rank within the same species crossing experiments have confirmed that their F1 hybrids are sterile. An analysis of twelve microsatellite markers, with unique alleles in each taxon, also shows exceedingly low levels of gene flow between populations of the two subspecies. Due to the lack of previous phylogenetic resolution among L. floccosa subspecies, we used Illumina next generation sequencing to identify single nucleotide polymorphisms from genomic DNA libraries of L. floccosa ssp. floccosa and L. floccosa ssp. grandiflora. These data were used to identify single nucleotide polymorphisms in the chloroplast, mitochondrial, and nuclear genomes. From these variable loci, a total of 2772 bp was obtained using Sanger sequencing of ten individuals representing all subspecies of L. floccosa and an outgroup....Continue Reading

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Datasets Mentioned

BETA
HQ179769
NC010323
NC012116

Methods Mentioned

BETA
Illumina sequencing
PCR

Software Mentioned

ABI GeneMapper
Modeltest
MrBayes
BioEdit
Velvet
PAUP
Primer3
RAxML
Mulan
MulTrees

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