PMID: 11337864May 8, 2001Paper

An evaluation of the etiology of reduced CYP1A1 messenger RNA expression in the Atlantic tomcod from the Hudson River, New York, USA, using reverse transcriptase polymerase chain reaction analysis

Environmental Toxicology and Chemistry
N K RoyI I Wirgin

Abstract

Adult Atlantic tomcod, Microgadus tomcod, from the Hudson River, New York State, USA, exhibit reduced inducibility of hepatic cytochrome P4501A1 (CYP1A1) mRNA compared with adult tomcod from the cleaner Miramichi River, New Brunswick, Canada, when treated with coplanar polychlorinated biphenyl (PCB) congeners or 2,3,7,8-tetrachlorodibenzo-p-dioxin. In contrast, little difference in CYP1A1 inducibility is observed between tomcod from these two rivers when treated with polycyclic aromatic hydrocarbons (PAHs). We sought to determine if impaired hepatic CYP1A1 inducibility in Hudson River tomcod results from a multigenerational, genetic adaptation or a single generational, physiological acclimation. Embryos and larvae from controlled experimental crosses of Hudson River and Miramichi River parents were exposed for 24 h to water-borne PCB congener 77 (10 ppm), benzo[a]pyrene (BaP; 10 ppm), or dimethysulfoxide, and CYP1A1 expression was assessed in individual larva using competitive reverse transcriptase polymerase chain reaction (RT-PCR) analysis. The CYP1A1 mRNA was significantly induced in larvae from both populations by BaP (47- and 52-fold) and PCB 77 (9- and 22-fold), although levels of expression were higher in offspring of Mi...Continue Reading

References

Feb 25, 1993·Nucleic Acids Research·F S CeliA R Shuldiner
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Sep 1, 1996·Toxicology and Applied Pharmacology·P M Fernandez-SalgueroF J Gonzalez
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Apr 1, 1998·Environmental Health Perspectives·M T LandiN E Caporaso
Jan 14, 1999·Genes & Development·J MimuraY Fujii-Kuriyama

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Citations

Jul 5, 2003·Aquatic Toxicology·Joshua P GrayJohn P Vanden Heuvel
Jan 6, 2006·Environmental Health Perspectives·Zhanpeng YuanIsaac Wirgin
May 7, 2015·Critical Reviews in Toxicology·T B Henry
Jul 10, 2002·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Nirmal K RoyIsaac Wirgin

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