An evaluation of the performance of XLD, DCA, MLCB, and ABC agars as direct plating media for the isolation of Salmonella enterica from faeces

Journal of Clinical Pathology
K J NyeR E Warren

Abstract

To compare the performance of four media, singly and in combination, as direct plating media for the isolation of Salmonella enterica from human faeces. Two thousand four hundred and nine routine, faecal samples received by four laboratories were inoculated on to xylose lysine desoxycholate (XLD), desoxycholate citrate (DCA), mannitol lysine crystal violet brilliant green (MLCB), and alpha-beta chromogenic (ABC) agars using standardised protocols, reagents, and data collection. Isolates of presumptive salmonellae were identified using standard laboratory techniques and the results were analysed statistically. Direct plating recovered 46 of the 60 possible isolates of Salmonella spp recovered via enrichment broth. No isolates were recovered from direct plating that were not recovered via selenite enrichment. MLCB gave the highest isolation rate individually (84.8%) and amounts of competing flora (CF) did not affect the recognition of colonies. ABC proved highly specific, but insensitive, and isolation rates were adversely affected by any amount of CF. Isolation rates from XLD and DCA were only affected when the CF load was heavy. DCA was least specific, with only 9.01% of picks positive and greatest number of confirmatory tests....Continue Reading

References

Sep 1, 1994·Journal of Clinical Pathology·C A Persaud, S J Eykyn

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Citations

Jan 1, 2009·Journal of Tropical Medicine·A SangwaiyaJ Arnold
Sep 26, 2013·The American Journal of Tropical Medicine and Hygiene·Andrew GoodyearSteven Dow
Nov 30, 2007·Journal of Applied Microbiology·J D Perry, A M Freydière
Feb 16, 2010·Marine Pollution Bulletin·Catarina R MansilhaAntónio Tavares
Feb 24, 2011·Journal of Nursing Scholarship : an Official Publication of Sigma Theta Tau International Honor Society of Nursing·Nikki L HillAndrea Yevchak

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