An evolutionary conserved element is essential for somite and adjacent mesenchymal expression of the Hoxa1 gene

Developmental Dynamics : an Official Publication of the American Association of Anatomists
J R ThompsonL J Gudas

Abstract

The murine Hoxa1 gene is a member of the vertebrate Hox complex and plays a role in defining the body plan during development. At day 8.0-9.0 post coitus, Hoxa1 transcripts are detected extensively throughout the embryo in the neural tube, adjacent mesenchyme, paraxial mesoderm, somites and gut epithelium; expression extends from the most caudal region of the embryo to the rhombomere 3/4 border. This spatiotemporal expression of Hoxa1 mRNA is critical for normal embryonic development. We have previously identified a 10 bp element, called CE2, which is located approximately 3 kilobases 3' of the Hoxa1 coding region in the RAIDR5 enhancer, and which binds to an approximately 170 kd protein in retinoic acid treated P19 embryonal carcinoma cells. CE2 elements were also identified 3' of the murine Hoxb1 gene, the chicken Hoxb1 gene and the human Hoxa1 gene. To examine the role of this CE2 element in regulating Hoxa1 expression in vivo, transgenic mice were generated which express a Hoxa1 beta-galactosidase reporter gene that contains a mutation in the CE2 element. Relative to transgenic mice bearing a wild type CE2 element, the mutant CE2 construct recapitulated rhombomeric, neural, and gut epithelium expression but failed to show b...Continue Reading

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Citations

Nov 23, 2010·The Journal of Biological Chemistry·Vasundhra KashyapJoseph M Scandura
Jan 28, 2003·American Journal of Medical Genetics. Part a·Julie E Hoover-FongA Hamosh
Mar 14, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Danyang HuangLorraine J Gudas
Mar 1, 2018·Theory in Biosciences = Theorie in Den Biowissenschaften·Takayuki Onai
Mar 17, 2010·The Journal of Biological Chemistry·Vasundhra Kashyap, Lorraine J Gudas
Jun 7, 2017·Proceedings of the National Academy of Sciences of the United States of America·Bony De KumarRobb Krumlauf
Feb 25, 1999·Gastroenterology·R K MontgomeryR J Grand
Sep 23, 2006·Neurotoxicology and Teratology·Christopher J StodgellPatricia M Rodier

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