An ex vivo culture model for orthodontically induced root resorption

Journal of Dentistry
W N Wan HassanA J Sloan

Abstract

Root resorption is a ubiquitous although undesirable sequela to orthodontic treatment. Current methods to investigate the pathophysiology have certain limitations. In pursuit to understand and develop treatment modalities for orthodontically induced root resorption, the ability to manipulate cells within their natural extracellular matrix in a three dimensional organotypic model is invaluable. The study aimed to develop a laboratory-based organotypic model to investigate the effect of orthodontic forces on the periodontium. Mandibular slices of male Wistar rats were maintained in Trowel-typed cultures at 37°C in 5% carbon dioxide in air for 7 days with test specimens subjected to compressive forces at 50 g and 100g by stainless steel springs. Tissue architecture and cell viability were maintained under culture conditions. Osteoclast numbers increased significantly in both test groups whilst odontoclasts increased in the 50 g group. Immunohistochemistry demonstrated increased dentine sialoprotein expression in both test groups, suggesting changes in mineralization-related activity due to mechanical strain. The study showed initial cellular and molecular changes of key markers that relate to root resorption in response to mechani...Continue Reading

References

Sep 1, 1975·Journal of Dentistry·P C Makkes, S K Thoden van Velzen
Mar 1, 1992·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·T W PoppL Linge
Jul 1, 1989·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·D N RemingtonM K Chapko
May 1, 1984·Journal of Dental Research·G W DuncanD M Suga
Jun 1, 1981·American Journal of Orthodontics·A F Barber, M R Sims
Jun 1, 1995·European Journal of Orthodontics·P Brudvik, P Rygh
Sep 30, 1993·Biochemical and Biophysical Research Communications·J Owens, T J Chambers
Apr 11, 1997·Science·S A Nesbitt, M A Horton
Aug 12, 1999·British Journal of Haematology·H M Massey, A M Flanagan
Mar 15, 2000·Archives of Oral Biology·A J SloanA J Smith
Jan 29, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Hiroyuki KanzakiHideo Mitani
May 15, 2002·Journal of Periodontal Research·R MabuchiM Shimono
Jul 5, 2002·Journal of Dental Research·C QinW T Butler
Dec 20, 2002·Connective Tissue Research·K DobieA J Smith
Jul 25, 2003·Microscopy Research and Technique·Takahisa Sasaki
Sep 4, 2003·Connective Tissue Research·Chunlin QinWilliam T Butler
Apr 2, 2004·European Journal of Oral Sciences·Otto BabaWilliam T Butler
Mar 8, 2005·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Eugene Chan, M Ali Darendeliler
Nov 30, 2005·Immunological Reviews·F Patrick Ross, Steven L Teitelbaum
Nov 14, 2006·Archives of Oral Biology·Laura BalducciAnne George
Nov 18, 2006·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Debora Alvares HarrisM Ali Darendeliler
Jun 26, 2007·Journal of Dental Research·K NakaoK Yamaguchi
Nov 27, 2007·Journal of Dental Research·D C WescottM C Meikle
Feb 27, 2008·International Endodontic Journal·P E MurrayP J Lumley
Nov 26, 2008·Journal of Dental Research·E M MullaneJ E Nör
Feb 7, 2009·European Journal of Oral Sciences·Rui XieJaap C Maltha
Apr 8, 2009·Matrix Biology : Journal of the International Society for Matrix Biology·Shigeki SuzukiAshok B Kulkarni
May 2, 2009·Journal of Dental Research·F W G Paula-SilvaY L Kapila
Oct 27, 2009·Experimental Cell Research·Barbro Ek-Rylander, Göran Andersson
Mar 12, 2010·Tissue Engineering. Part C, Methods·Emma L SmithAlastair J Sloan
Aug 23, 2011·Journal of Dentistry·T El-BialyA J Sloan

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Citations

Jun 28, 2017·Ultrasound in Medicine & Biology·Khuloud S AlhazmiAli H Hassan
Aug 3, 2020·Journal of Cellular Physiology·Tiancheng LiPeipei Duan
Feb 6, 2020·International Journal of Molecular Sciences·Alexander SieberathKenny Dalgarno
Jan 26, 2021·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Tiancheng LiPeipei Duan
Feb 5, 2021·Frontiers in Bioengineering and Biotechnology·Reem El-GendyJennifer Kirkham

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