An ex vivo Model of HIV-1 Infection in Human Lymphoid Tissue and Cervico-vaginal Tissue

Bio-protocol
Andrea IntroiniLeonid Margolis

Abstract

Human tissue explants are a valuable tool to study the interactions between host and infectious agents. They reliably mimic many important aspects of tissue cytoarchitecture and functions and allow us the investigation of the mechanisms of microbial pathogenesis under controlled laboratory conditions. One of the advantages of this system is that, unlike isolated cells, infection of tissue blocks with HIV-1 does not require exogenous stimulation with mitogens or activating factors. Here we describe a protocol to infect with HIV-1 human lymphoid tissue from tonsils and cervico-vaginal tissue and maintain them in culture in a non-polarized setting. These ex vivo infected tissues can be used as fruitful models to study HIV-1 pathogenesis and HIV-1 vaginal transmission, respectively, as well as an efficient platform for testing anti-HIV therapeutic and preventative strategies.

References

Dec 23, 2008·Journal of Virological Methods·Angélique BiancottoJean-Charles Grivel
Feb 7, 2009·Nature Protocols·Jean-Charles Grivel, Leonid Margolis
Jan 13, 2011·American Journal of Reproductive Immunology : AJRI·Melanie MerbahLeonid Margolis
Dec 12, 2012·Current HIV/AIDS Reports·Charlene S Dezzutti, Florian Hladik
Feb 15, 2013·PLoS Pathogens·Andrea IntroiniLeonid Margolis

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Citations

Jun 6, 2017·Frontiers in Microbiology·Rogers A Ñahui PalominoLeonid Margolis
Aug 3, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dominic Paquin-ProulxJohan K Sandberg
Mar 15, 2019·Journal of Virology·Jessica Katy SkeltonMarcus Dorner
Nov 5, 2020·The EMBO Journal·Sheetal KawOliver T Fackler
Aug 24, 2016·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Giulia CalendaNatalia Teleshova

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