PMID: 8944770Nov 1, 1996Paper

An example of non-conservation of oligomeric structure in prokaryotic aminoacyl-tRNA synthetases. Biochemical and structural properties of glycyl-tRNA synthetase from Thermus thermophilus

European Journal of Biochemistry
M H MazauricD Kern

Abstract

Glycyl-tRNA synthetase (Gly-tRNA synthetase) from Thermus thermophilus was purified to homogeneity and with high yield using a five-step purification procedure in amounts sufficient to solve its crystallographic structure [Logan, D.T., Mazauric, M.-H., Kern, D. & Moras, D. (1995) EMBO J. 14, 4156-4167]. Molecular-mass determinations of the native and denatured protein indicate an oligomeric structure of the alpha 2 type consistent with that found for eukaryotic Gly-tRNA synthetases (yeast and Bombyx mori), but different from that of Gly-tRNA synthetases from mesophilic prokaryotes (Escherichia coli and Bacillus brevis) which are alpha 2 beta 2 tetramers. N-terminal sequencing of the polypeptide chain reveals significant identity, reaching 50% with those of the eukaryotic enzymes (B. mori, Homo sapiens, yeast and Caenorhabditis elegans) but no significant identity was found with both alpha and beta chains of the prokaryotic enzymes (E. coli, Haemophilus influenzae and Coxiella burnetii) albeit the enzyme is deprived of the N-terminal extension characterizing eukaryotic synthetases. Thus, the thermophilic Gly-tRNA synthetase combines strong structural homologies of eukaryotic Gly-tRNA synthetases with a feature of prokaryotic syn...Continue Reading

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Citations

Mar 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·James H CampbellMircea Podar
Jan 23, 1999·Current Opinion in Genetics & Development·R F Doolittle, J Handy
Feb 17, 2015·Fungal Genetics and Biology : FG & B·Chin-I ChienChien-Chia Wang
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Jan 23, 2016·The Journal of Biological Chemistry·Xiangyu DengWei Xie
May 27, 2016·The Journal of Biological Chemistry·Marco Igor Valencia-SánchezAlfredo Torres-Larios
Nov 18, 2018·PLoS Computational Biology·Joana C XavierIsabel Rocha
Jun 15, 2016·The Journal of Biological Chemistry·Chih-Yao ChangChien-Chia Wang
Jan 20, 1998·Trends in Biochemical Sciences·K ShibaP Schimmel
May 7, 2003·Biochemistry·John David DignamJonathan B Chaires

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