An exclusively nuclear RNA-binding protein affects asymmetric localization of ASH1 mRNA and Ash1p in yeast

The Journal of Cell Biology
Roy M LongPascal Chartrand

Abstract

The localization of ASH1 mRNA to the distal tip of budding yeast cells is essential for the proper regulation of mating type switching in Saccharomyces cerevisiae. A localization element that is predominantly in the 3'-untranslated region (UTR) can direct this mRNA to the bud. Using this element in the three-hybrid in vivo RNA-binding assay, we identified a protein, Loc1p, that binds in vitro directly to the wild-type ASH1 3'-UTR RNA, but not to a mutant RNA incapable of localizing to the bud nor to several other mRNAs. LOC1 codes for a novel protein that recognizes double-stranded RNA structures and is required for efficient localization of ASH1 mRNA. Accordingly, Ash1p gets symmetrically distributed between daughter and mother cells in a loc1 strain. Surprisingly, Loc1p was found to be strictly nuclear, unlike other known RNA-binding proteins involved in mRNA localization which shuttle between the nucleus and the cytoplasm. We propose that efficient cytoplasmic ASH1 mRNA localization requires a previous interaction with specific nuclear factors.

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D St JohnstonM Jantsch
Nov 11, 1991·Nucleic Acids Research·Y H ZhouR H Ebright
Jul 12, 1991·Cell·D St JohnstonC Nüsslein-Volhard
Dec 1, 1994·Molecular and Cellular Biology·J FlachP A Silver
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·L LiR J Legerski
Nov 1, 1993·The Journal of Cell Biology·G SchlenstedtP A Silver
Apr 1, 1993·Current Opinion in Genetics & Development·K Nasmyth
Jul 1, 1996·Nucleic Acids Research·U GüldenerJ H Hegemann
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·D J SenGuptaM Wickens
Apr 1, 1997·Molecular and Cellular Biology·A F RossR H Singer
Nov 11, 1998·Molecular Cell·E BertrandR M Long
Jun 9, 1999·Current Biology : CB·D L BeachK Bloom
May 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·P A Takizawa, R D Vale
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·M E Maxon, I Herskowitz

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Citations

Dec 14, 2011·Cellular and Molecular Life Sciences : CMLS·Roland Gerhard Heym, Dierk Niessing
Jul 28, 2006·Molecular Genetics and Genomics : MGG·Carl R UrbinatiRoy M Long
Sep 5, 2007·Molecular Genetics and Genomics : MGG·Kathi Zarnack, Michael Feldbrügge
Dec 10, 2003·Current Opinion in Microbiology·Xavier DarzacqDaniel Zenklusen
Sep 6, 2003·Gene·Micheline Fromont-RacineFranco Fasiolo
Nov 21, 2002·Trends in Cell Biology·Kim L Farina, Robert H Singer
Apr 27, 2005·Nature Reviews. Molecular Cell Biology·Daniel St Johnston
Jul 3, 2013·Nature Structural & Molecular Biology·Thomas E SladewskiKathleen M Trybus
Dec 17, 2004·Biology of the Cell·Graydon B GonsalvezRoy M Long
Sep 13, 2001·Molecular Biology of the Cell·D L Beach, K Bloom
Aug 6, 2008·Molecular and Cellular Biology·Francisco Malagon, Torben Heick Jensen
Apr 15, 2004·Molecular and Cellular Biology·Tomofumi TadauchiKenji Irie
Nov 1, 2001·Annual Review of Cell and Developmental Biology·P ChartrandR M Long
Apr 24, 2007·RNA·Ashwini Jambhekar, Joseph L Derisi
Apr 22, 2004·RNA·Edward W HorseyJohn L Woolford
Sep 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·K A ShepardR D Vale
Dec 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Annika NiednerDierk Niessing
Jan 24, 2013·Genetics·Wioletta CzajaBruce L Miller
Oct 11, 2011·Cellular and Molecular Life Sciences : CMLS·Karen Shahbabian, Pascal Chartrand
Aug 27, 2004·Médecine sciences : M/S·Eugenia BasyukEdouard Bertrand
May 12, 2010·The Journal of Cell Biology·Sunglan Chung, Peter A Takizawa
Apr 21, 2004·The Journal of Cell Biology·Tracy L KressKimberly L Mowry
Mar 6, 2002·The Journal of Cell Biology·Laura R SchenkmanJohn R Pringle
Dec 24, 2014·Proceedings of the National Academy of Sciences of the United States of America·Nimisha SinghHang Shi
Sep 10, 2015·Proceedings of the National Academy of Sciences of the United States of America·Jing YangHao Li
Feb 12, 2008·Trends in Cell Biology·Nicolas Paquin, Pascal Chartrand
Apr 27, 2007·Seminars in Cell & Developmental Biology·Corinna Giorgi, Melissa J Moore
Jul 30, 2004·The EMBO Journal·Oliver Rackham, Chris M Brown
Sep 29, 2005·Biochemical and Biophysical Research Communications·Viviana VoltaStefano Biffo
Mar 25, 2014·Seminars in Cell & Developmental Biology·Piergiorgio Percipalle
Sep 6, 2003·Current Biology : CB·Robert H Singer
Mar 7, 2002·Developmental Cell·Elissa P Lei, Pamela A Silver

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Methods Mentioned

BETA
PCR
three-hybrid
Protein Shuttling
in vitro transcription
electrophoresis
affinity purification
RNA
immunoprecipitation
mobility
immunodepletion

Software Mentioned

Scanalytics
Esprit Image Analysis
Adobe Photoshop

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