PMID: 698224Aug 23, 1978Paper

An exonuclease activity associated with DNA polymerase I of Micrococcus radiodurans

Biochimica Et Biophysica Acta
S KitayamaA Matsuyama

Abstract

An exonuclease activity is associated with one of three DNA polymerase in Micrococcus radiodurans. The nuclease activity co-sedimented with its DNA polymerase I of this bacterium on glycerol gradient centrifugation. Both activities show the same optimum pH and heat-inactivation kinetics. This nuclease hydrolyzes preferentially double-stranded DNA in an exonucleolytic manner from both ends of the duplex DNA. The products of hydrolysis are mostly deoxyribonucleoside 5'-monophosphate and no nucleosides are released into the acid-soluble fraction. Di- or other oligonucleotides are also produced but their relative amounts are constant during the time of incubation. The exonuclease activity requires Mg2+ and is inhibited by high concentrations of KCl as is DNA polymerase I of M. radiodurans.

References

Mar 2, 1977·Biochimica Et Biophysica Acta·S Kitayama, A Matsuyama
Feb 5, 1976·Biochimica Et Biophysica Acta·S Kitayama, A Matsuyama
Feb 21, 1967·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·J T LettC J Dean
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·E B Konrad, I R Lehman
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Jan 1, 1968·Cold Spring Harbor Symposia on Quantitative Biology·E Cerdá-Olmedo, P C Hanawalt
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Jan 1, 1971·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·S Kitayama, A Matsuyama
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Nov 8, 1968·Biochemical and Biophysical Research Communications·S Kitayama, A Matsuyama

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