An exosomal- and interfacial-biosensing based strategy for remote monitoring of aberrantly phosphorylated proteins in lung cancer cells

Biomaterials Science
Mostak AhmedMatt Trau

Abstract

It is a well-known phenomenon that cancer cells release key biological information such as DNA, RNA or proteins into body fluids (e.g., blood, urine or saliva). The analysis of these molecules-often encapsulated within nanovesicules called exosomes-is highly attractive, because it could replace current surgical biopsies, which are painful, costly and potentially risky for patients. For example, current strategies in lung cancer diagnosis involve genetic analyses from tumour tissues to detect the presence of underlying DNA mutations, known to alter the phosphorylation status and function of proteins. This information is used to direct therapy, as aberrantly phosphorylated proteins are the main targets of current drugs such as Tyrosine Kinase Inhibitors (TKIs). An alternative and less invasive strategy would be the remote analysis of these phospho-proteins by isolating them from cancer-derived exosomes. This would allow evaluating not only their phosphorylation status at diagnosis, but also the timely restoration of protein phosphorylation levels during therapy with TKIs. Yet, this proteomic approach remains vastly unexplored. Herein, we demonstrate that key lung cancer phosphoproteins, such as EGFR and ERK, are expressed in lung...Continue Reading

References

May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S B KannerJ T Parsons
Mar 9, 2000·Journal of Pharmaceutical and Biomedical Analysis·J W FindlayR R Bowsher
Aug 2, 2002·The Journal of Biological Chemistry·Hui ZhangMichael J Comb
Nov 28, 2002·Nature Genetics·Gavin MacBeath
Mar 11, 1953·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·D P RILEY, U W ARNDT
Apr 17, 2004·American Journal of Respiratory and Critical Care Medicine·Alain C BorczukCharles A Powell
Feb 22, 2005·Methods : a Companion to Methods in Enzymology·Benjamin A GarciaDonald F Hunt
Jun 23, 2009·Journal of Immunological Methods·Andrew N Hoofnagle, Mark H Wener
Dec 5, 2009·Clinical Chemistry·Allison A EllingtonGeorge G Klee
Apr 9, 2014·Cell Research·Basant Kumar ThakurDavid Lyden
Aug 26, 2014·Frontiers in Genetics·Hafumi NishiAnna R Panchenko
Jan 14, 2017·Cancers·Young Hwa SoungJun Chung

❮ Previous
Next ❯

Citations

Sep 19, 2019·Advanced Materials·Phuong H L TranWei Duan
Sep 16, 2020·Analytical Methods : Advancing Methods and Applications·Houman Kholafazad Kordasht, Mohammad Hasanzadeh
Feb 24, 2020·Seminars in Cancer Biology·Christopher WoodmanCornelia M Wilson
Jun 27, 2019·Molecular Pharmaceutics·Liangdi JiangJiyong Liu
Feb 26, 2019·ACS Applied Materials & Interfaces·Mostak AhmedMatt Trau

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
immunoprecipitation
biopsy
biosensing
chip

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.