PMID: 6169011Aug 11, 1981Paper

An experimental approach to enumerate the genes coding for immunoglobulin variable-regions

Nucleic Acids Research
E P ZeelonI Schechter

Abstract

Critical to our understanding of the immune system diversity is the determination of the number of germ line V genes. The total number of V genes is given by the product: number of subgroups x number of germ line genes per subgroup. Studies of kappa chains and of embryonic DNA indicate 5-10 V genes per subgroup. Statistical analysis of the limited sequence data of mouse kappa chains suggest about 50 V kappa subgroups. We report here a general approach for direct estimation of the number of VL and VH subgroups expressed in normal spleen, and present data for V kappa. The kappa mRNA of the spleen is a heterogeneous population where different V kappa are linked to the same C kappa, i.e. C kappa equals total V kappa. The ratio C kappa/distinct V kappa approximates the number of subgroups since V kappa of the same subgroup cross hybridize while V kappa of different subgroups do not. This ratio was determined by molecular hybridization of cloned C kappa and V kappa DNA probes with spleen mRNA. The results indicate the expression of 280 V kappa subgroups in mouse. Assuming an average of 7 genes per subgroup, we estimate about 2000 V kappa germ line genes.

References

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Citations

Jan 1, 1986·Immunogenetics·L A D'Hoostelaere, D M Gibson
Jan 1, 1989·Immunogenetics·R KoflerF J Dixon
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·Y BursteinI Schechter
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S K SikderS L Morrison
Dec 1, 1987·Immunology Letters·R KoflerR Fässler
Jun 20, 1987·Journal of Molecular Biology·K H LeeT Honjo
Jan 1, 1988·Scandinavian Journal of Rheumatology. Supplement·A N TheofilopoulosP Singer

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