An Experimental Inoculation System to Study Citrus-Xylella fastidiosa Interactions

Plant Disease
Silvio LopesW B Li

Abstract

Difficulties in reproducing the citrus variegated chlorosis (CVC) disease symptoms in experimental plants have delayed implementation of studies to better understand the essential aspects of this important disease. In an extensive study, cultivars of sweet orange (Citrus sinensis) were inoculated with Xylella fastidiosa using procedures that included root immersion, and stem absorption, pricking, or infiltration of the inoculum into plants of different ages. Inoculum consisted of 5-day-old cultures or cell suspensions of CVC strain 9a5c diluted in phosphate-buffered saline. Inoculated plants and controls were grown, or transferred just after inoculation, to 5-liter pots or 72-cell foam trays. Approximately 4, 5, 9, and 12 months after inoculation, leaves were collected and processed for polymerase chain reaction analysis or X. fastidiosa isolation on BCYE agar medium. Root immersion and stem inoculation of 4- and 6-month-old plants resulted in low percentages of symptomatic (0 to 7%) and plants positive by isolation (0 to 9%). Pinpricked or injected stems of 1-month-old seedlings resulted in high percentages of plants symptomatic (29 and 90% in Pera Rio, 75, 59, and 83% in Valencia, and 77% in Natal) or positive by isolation (2...Continue Reading

Citations

Jul 23, 2018·EFSA Journal·UNKNOWN EFSA Panel on Plant Health (EFSA PLH Panel)Claude Bragard
May 8, 2008·Current Microbiology·Nelson Arno WulffPatrícia Brant Monteiro
Jun 24, 2010·Biosecurity and Bioterrorism : Biodefense Strategy, Practice, and Science·Veronica AnconaPaul de Figueiredo
Sep 1, 2012·Plant Disease·A L GarciaS A Lopes
May 15, 2019·EFSA Journal·UNKNOWN EFSA Panel on Plant Health (PLH)Stephen Parnell

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.