An experimental model for exudative age-related macular degeneration with choroidal neovascularization using the common marmoset

Current Neurovascular Research
Masamitsu ShimazawaHideaki Hara

Abstract

This study aimed to establish an experimental exudative age-related macular degeneration (AMD) model in the common marmoset (Callithrix jacchus), which is a small New World monkey. Choroidal neovascularization (CNV) was induced by laser irradiation on the left eye of each animal under anesthesia. Eight laser spots were applied around the macular area using the image-guided laser system (532 nm) attached with Micron III at 650 mW-2,000 mW power. Laser pulse duration and spot size were fixed at 100 ms and 50 µm, respectively. At 14 days after laser irradiation, fluorescein angiograms were observed. At 21 days after laser irradiation, the fluorescein angiograms were transcardially perfused to the bilateral common carotid arteries with 4% paraformaldehyde for the transverse section or with fluorescein-conjugated dextran (MW = 2,000 kDa) for the retinal pigment epithelia (RPE)-choroidal flatmount. At 14 days after laser irradiation, late hyperfluorescence and leakage within or beyond the lesion borders were observed in a laser power-dependent manner. In the RPE-choroidal flatmount, the mean size of the CNV lesions at 1,500 mW was 1.34 ± 0.49 × 10(5) µm(2) (Mean ± S.D., n = 29), and the coefficient of variation for each CNV area was ...Continue Reading

Citations

Sep 7, 2018·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Masamitsu Shimazawa, Hideaki Hara
Jan 6, 2017·Oxidative Medicine and Cellular Longevity·Yuhei Nishimura, Hideaki Hara
May 6, 2019·Investigative Ophthalmology & Visual Science·Shinsuke NakamuraMasamitsu Shimazawa

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