An exploration of nucleic acid liquid biopsy using a glucose meter

Chemical Science
Yu GuHong-Yuan Chen

Abstract

The development of non-invasive techniques for the diagnosis of cancer, characterization of mutation and monitoring treatment response could greatly reduce the morbidity and mortality caused by cancer. Nevertheless, the extremely low amount of cell free nucleic acids makes liquid biopsy a very challenging task. Herein, taking advantage of the pocket size, reliable quantitative results and simple operation of the pocket-sized personal glucose meter (PGM), we report an approach of circulating microRNA-21 (miR-21) detection with high precision and low cost. Via target-induced release of invertase from the DNA-invertase conjugate, which could convert sucrose into glucose, the detection of miR-21 in serum was linked to PGM readings. Combining the DNAzyme feedback amplification (DFA) program and highly efficient enzymatic turnover, an ultralow detection limit of 7 × 10-16 M for miR-21 was achieved using a PGM as the reporter. The high sensitivity and selectivity of the proposed method meets the requirement of quantifying cell free nucleic acids in serum. In addition, this approach fills the shortage of quantitative RT-PCR and next-generation sequencing in quantifying miRNAs with a short length and greatly reduces the cost of detectio...Continue Reading

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Citations

Oct 16, 2019·Nature Reviews. Endocrinology·Chenbo Ji, Xirong Guo
Aug 29, 2020·Pharmaceutics·Alberto JuanCarlos Alonso-Moreno
Oct 4, 2018·Analytical and Bioanalytical Chemistry·Linan ZhangLihua Li
Oct 1, 2020·Sensors·Clara Abardía-SerranoMaría Jesús Lobo-Castañón
Sep 19, 2019·ACS Applied Materials & Interfaces·Piaopiao ChenJia Geng

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Methods Mentioned

BETA
biopsy
nucleic
reverse transcription-PCR
PCR
nucleic acid assay
electrophoresis
atomic force microscopy
AFM
FRET

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