An expression system based on the promoter region of the Aspergillus awamori 1,4-beta-endoxylanase A gene

Applied Microbiology and Biotechnology
R J GoukaC A Van den Hondel

Abstract

A new, highly inducible fungal promoter derived from the Aspergillus awamori 1,4-beta-endoxylanase A (exlA) gene is described. Induction analysis, carried out with the wild-type strain in shake flasks, showed that exlA expression in regulated at the transcriptional level. Using a beta-glucuronidase (uidA) reporter strategy, D-xylose was shown to be an efficient inducer of the exlA promoter, whereas sucrose or maltodextrin were not. Upon D-xylose induction, the exlA promoter was threefold more efficient than the frequently used A. niger glucoamylase (glaA) promoter under maltodextrin induction. Detailed induction analyses demonstrated that induction was dependent on the presence of D-xylose in the medium. Carbon-source-limited chemostat cultures with the uidA reporter strain showed that D-xylose was also a very good inducer in a fermenter, even in the presence of sucrose.

Citations

Jun 10, 2010·Applied Microbiology and Biotechnology·André Fleissner, Petra Dersch
Apr 12, 2002·Trends in Biotechnology·Peter J PuntCees van den Hondel
Jun 20, 2003·Journal of Biotechnology·B Christien LokmanCees A M J J van den Hondel
May 26, 2001·Applied and Environmental Microbiology·R J GoukaC T Verrips
Feb 11, 2014·Physics of Life Reviews·Ana Leonor RiveraAchim M Loske
Sep 21, 2004·Applied Microbiology and Biotechnology·Vivi JoostenB Christien Lokman
Aug 28, 2014·Critical Reviews in Microbiology·Monika Sharma, Rohit Sharma
Jan 1, 1997·Critical Reviews in Biotechnology·D B Archer, J F Peberdy
Apr 4, 2000·Biotechnology and Bioengineering·M G WiebeA P Trinci
May 3, 2008·International Journal of Systematic and Evolutionary Microbiology·Jianli ZhangFei Song
Jun 4, 2018·Applied Microbiology and Biotechnology·Janina KlugeUlrich Kück
Nov 19, 2011·FEMS Microbiology Letters·Ruchika SharmaGhulam Nabi Qazi
Aug 4, 1998·Applied and Environmental Microbiology·I A van GemerenC T Verrips
Oct 9, 2008·Biotechnology Advances·David Lubertozzi, Jay D Keasling

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.