PMID: 11342249May 9, 2001Paper

An extracellular beta-galactofuranosidase from Aspergillus niger and its use as a tool for glycoconjugate analysis

Biochimica Et Biophysica Acta
G L WallisJ F Peberdy

Abstract

Aspergillus niger produces an extracellular beta-galactofuranosidase, which can specifically hydrolyse beta-D-galactofuranose (Galf) from glycoconjugates. The production of this enzyme can be induced by the addition of a Galf-containing A. niger mycelial wall extract. However, on other carbon sources accumulation occurred only during the starvation conditions of the late stationary phase. Extracellular glucoamylases from this stage of cultivation possessed significantly lower levels of Galf than those from the earlier exponential growth phase when beta-galactofuranosidase is absent, suggesting in situ beta-galactofuranosidic hydrolysis. The beta-galactofuranosidase responsible was subsequently purified to homogeneity and characterised. It is a glycoprotein of 90 kDa (determined by SDS-PAGE) with activity against beta-linked Galf residues, with a Km of 4 mM against p-nitrophenyl-beta-D-galactofuranoside and a pH optimum of 3-4. The preparation did not contain other contaminating glycosidase activities; p-nitrophenyl-beta-D- and -alpha-D-galactopyranose, and alpha-D-methyl-Galf were not hydrolysed. Results are presented to show that this enzyme could be employed as a useful tool for the analysis of glycoconjugates containing biol...Continue Reading

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Oct 1, 1995·Bioscience, Biotechnology, and Biochemistry·N RamliS Iwahara
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Oct 6, 1997·Archives of Biochemistry and Biophysics·G L WallisJ F Peberdy
Jan 1, 1997·Critical Reviews in Biotechnology·D B Archer, J F Peberdy
Nov 24, 1999·Biochimica Et Biophysica Acta·G L WallisJ F Peberdy

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Citations

Mar 5, 2003·Molecular and Biochemical Parasitology·Luiz Claudio MilettiRosa M de Lederkremer
Jul 11, 2001·FEMS Microbiology Letters·G L WallisJ F Peberdy
Aug 8, 2001·European Journal of Biochemistry·G L WallisJ F Peberdy
Sep 24, 2011·Glycobiology·Boris TefsenFrançoise H Routier
Jul 9, 2005·Eukaryotic Cell·W MorelleJ-P Latgé
Jun 3, 2004·The Lancet Infectious Diseases·Monique A S H Mennink-KerstenPaul E Verweij
Jun 26, 2007·Bioscience, Biotechnology, and Biochemistry·Masatoshi Goto
May 20, 2020·International Journal of Molecular Sciences·Mateja SeničarRichard Daniellou
Apr 19, 2012·Natural Product Reports·Pierre Lafite, Richard Daniellou
Oct 5, 2020·Journal of Bioscience and Bioengineering·Emiko MatsunagaKaoru Takegawa

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