An extracellular pathway for dystroglycan function in acetylcholine receptor aggregation and laminin deposition in skeletal myotubes.

The Journal of Biological Chemistry
Mathieu R Tremblay, Salvatore Carbonetto

Abstract

The dystroglycan (DG) complex is involved in agrin-induced acetylcholine receptor clustering downstream of muscle-specific kinase where it regulates the stability of acetylcholine receptor aggregates as well as assembly of the synaptic basement membrane. We have previously proposed that this entails coordinate extracellular and intracellular interactions of its two subunits, alpha- and beta-DG. To assess the contribution of the extracellular and intracellular portions of DG, we have used adenoviruses to express full-length and deletion mutants of beta-DG in myotubes derived from wild-type embryonic stem cells or from cells null for DG. We show that alpha-DG is properly glycosylated and targeted to the myotube surface in the absence of beta-DG. Extracellular interactions of DG modulate the size and the microcluster density of agrin-induced acetylcholine receptor aggregates and are responsible for targeting laminin to these clusters. Thus, the association of alpha- and beta-DG in skeletal muscle may coordinate independent roles in signaling. We discuss how DG may regulate synapses through extracellular signaling functions of its alpha subunit.

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Citations

Aug 21, 2013·PloS One·Eric K JohnsonFederica Montanaro
Sep 6, 2012·Developmental Neurobiology·Huashan PengSalvatore Carbonetto
Mar 29, 2011·Neuromuscular Disorders : NMD·Clarissa M ComimJoão Quevedo
Jan 2, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Paola ConcolinoFrancesca Sciandra
Jun 22, 2017·Development·Jérome Chal, Olivier Pourquié
Aug 28, 2019·The Journal of Comparative Neurology·Geoffroy NoëlHakima Moukhles
Oct 2, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Armin AkhavanJohn L Muschler
Dec 15, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Raphael Vezina-AudetteSalvatore Carbonetto

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