An extragenic suppressor of prp24-1 defines genetic interaction between PRP24 and PRP21 gene products of Saccharomyces cerevisiae

Molecular & General Genetics : MGG
V C VaidyaU Vijayraghavan

Abstract

The temperature-sensitive prp24-1 mutation defines a gene product required for the first step in pre-mRNA splicing. PRP24 is probably a component of the U6 snRNP particle. We have applied genetic reversion analysis to identify proteins that interact with PRP24. Spontaneous revertants of the temperature-sensitive (ts)prp24-1 phenotype were analyzed for those that are due to extragenic suppression. We then extended our analysis to screen for suppressors that confer a distinct conditional phenotype. We have identified a temperature-sensitive extragenic suppressor, which was shown by genetic complementation analysis to be allelic to prp21-1. This suppressor, prp21-2, accumulates pre-mRNA at the non-permissive temperature, a phenotype similar to that of prp21-1. prp21-2 completely suppresses the splicing defect and restores in vivo levels of the U6 snRNA in the prp24-1 strain. Genetic analysis of the suppressor showed that prp21-2 is not a bypass suppressor of prp24-1. The suppression of prp24-1 by prp21-2 is gene specific and also allele specific with respect to both the loci. Genetic interactions with other components of the pre-spliceosome have also been studied. Our results indicate an interaction between PRP21, a component of t...Continue Reading

References

Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J Jarvik, D Botstein
Apr 1, 1991·Genes & Development·E J Strauss, C Guthrie
Mar 1, 1991·Trends in Genetics : TIG·S W Ruby, J Abelson
Sep 1, 1989·Molecular and Cellular Biology·J Banroques, J N Abelson
Aug 1, 1989·Genes & Development·U VijayraghavanJ Abelson
Nov 1, 1989·Yeast·J L Woolford
Jan 1, 1986·Cold Spring Harbor Symposia on Quantitative Biology·C T Caskey
Jul 11, 1988·Nucleic Acids Research·W J DowerC W Ragsdale
Jan 1, 1986·American Journal of Medical Genetics·P A JacobsT Webb
Jan 1, 1970·Molecular & General Genetics : MGG·L H HartwellJ R Warner
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Jan 25, 1983·Nucleic Acids Research·J C Larkin, J L Woolford
Apr 1, 1994·Current Opinion in Genetics & Development·A J Newman
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J E Arenas, J N Abelson

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