Abstract
Identification of the immunochemical and structural properties of pathogenic anti-DNA antibodies is a major goal for understanding their origins and the mechanisms whereby they induce tissue lesions. Herein, we report on the production of an IgG2a,k anti-DNA monoclonal antibody (4B1), derived from a 12-month-old (NZB x NZW)F1 lupus mouse, able to form glomerular immune deposits. mAb 4B1 is a polyspecific antibody able to bind to ssDNA, actin, tubulin, cardiolipin and to laminin as shown by solid phase ELISAs. Indirect immunofluorescence labeling of HEp-2 cells gave a cytoplasmic staining pattern similar to that obtained with anti-cytoskeleton antibodies. Western blot analysis demonstrated that mAb 4B1 bore idiotype D23, previously shown to be characteristic of natural antibodies derived from normal mice. After injecting the 4B1-secreting hybridoma intraperitoneally into normal (NZW x BALB/c)F1 mice, glomerular immune deposits were observed along the capillary wall. These deposits contained mainly IgM, IgG2a and mAb 4B1, as demonstrated by direct immunofluorescence using a biotinylated-rat anti-4B1 idiotype mAb and kidney eluate analysis. Nucleotide sequence analysis of the VH and VL genes showed that mAb 4B1 is encoded by VH Q5...Continue Reading
References
Aug 1, 1976·The Journal of Experimental Medicine·S IzuiP A Miescher
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Dec 1, 1992·Kidney International·R M TermaatJ H Berden
Jun 1, 1992·Kidney International·D V VlahakosM P Madaio
Sep 1, 1992·The Journal of Experimental Medicine·D M TillmanT N Marion
Apr 1, 1991·Journal of Autoimmunity·B HentatiB Payelle-Brogard
Jan 24, 1991·Nature·J EriksonM Weigert
Feb 1, 1990·The Journal of Clinical Investigation·B P TsaoB H Hahn
Apr 1, 1990·Journal of Autoimmunity·J SabbagaM P Madaio
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R BaccalaS Avrameas
Jan 1, 1989·European Journal of Immunology·J SabbagaM P Madaio
Jun 1, 1987·The Journal of Experimental Medicine·D M Klinman, A D Steinberg
Jul 1, 1985·European Journal of Immunology·P LymberiS Avrameas
Oct 1, 1967·The Journal of Experimental Medicine·D KofflerH G Kunkel
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L JacobD Louvard
Jan 28, 1983·Journal of Immunological Methods·E Lamoyi, A Nisonoff
Jul 1, 1982·The Journal of Experimental Medicine·D R Abrahamson, J P Caulfield
Apr 1, 1980·Arthritis and Rheumatism·F Ebling, B H Hahn
Feb 1, 1993·Molecular Immunology·S TakahashiM Kyogoku
Feb 1, 1993·The Journal of Clinical Investigation·M S KatzM P Madaio
Citations
Aug 1, 1997·Molecular Immunology·F BrardF Tron
Jun 1, 1997·Thyroid : Official Journal of the American Thyroid Association·R S McIntosh, A P Weetman
Oct 18, 2000·Lupus·G SannaA Mathieu
Mar 16, 2004·Immunological Investigations·Ljiljana A DimitrijevićIrena Zivković
Dec 23, 1998·European Journal of Immunology·C P QuanJ P Bouvet
Apr 21, 2005·Rheumatology·B QiaoS D Xiong