An IFNγ/CXCL2 regulatory pathway determines lesion localization during EAE

Journal of Neuroinflammation
Joshua S StoolmanBenjamin M Segal

Abstract

Myelin oligodendrocyte glycoprotein (MOG)-reactive T-helper (Th)1 cells induce conventional experimental autoimmune encephalomyelitis (cEAE), characterized by ascending paralysis and monocyte-predominant spinal cord infiltrates, in C57BL/6 wildtype (WT) hosts. The same T cells induce an atypical form of EAE (aEAE), characterized by ataxia and neutrophil-predominant brainstem infiltrates, in syngeneic IFNγ receptor (IFNγR)-deficient hosts. Production of ELR+ CXC chemokines within the CNS is required for the development of aEAE, but not cEAE. The cellular source(s) and localization of ELR+ CXC chemokines in the CNS and the IFNγ-dependent pathways that regulate their production remain to be elucidated. The spatial distribution of inflammatory lesions and CNS expression of the ELR+ CXC chemokines, CXCL1 and CXCL2, were determined via immunohistochemistry and/or in situ hybridization. Levels of CXCL1 and CXCL2, and their cognate receptor CXCR2, were measured in/on leukocyte subsets by flow cytometric and quantitative PCR (qPCR) analysis. Bone marrow neutrophils and macrophages were cultured with inflammatory stimuli in vitro prior to measurement of CXCL2 and CXCR2 by qPCR or flow cytometry. CNS-infiltrating neutrophils and monocytes...Continue Reading

References

Jun 1, 1996·Brain : a Journal of Neurology·J W ThorpeD H Miller
Dec 12, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·C L Bone-LarsonS L Kunkel
May 3, 2002·Clinical and Experimental Immunology·M SchwarzH H Radeke
Feb 26, 2003·American Journal of Respiratory Cell and Molecular Biology·Bo-Chin ChiuStephen W Chensue
Jul 10, 2003·Lancet Neurology·Jun-ichi Kira
Jan 28, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Karthik VenkateshRoman J Giger
Feb 8, 2005·Journal of the Neurological Sciences·Benjamin M Segal
May 5, 2007·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Xisheng WangYasuhiro Suzuki
Dec 29, 2007·The Journal of Pathology·X Zhang, D M Mosser
Feb 19, 2008·Nature Medicine·Ingunn M StromnesJoan M Goverman
Oct 15, 2008·The Journal of Experimental Medicine·Jason R LeesJohn H Russell
Jun 12, 2010·European Journal of Immunology·Mark A KroenkeBenjamin M Segal
Jul 16, 2010·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Xiangshu WenYasuhiro Suzuki
Oct 19, 2010·Brain, Behavior, and Immunity·Mark A Kroenke, Benjamin M Segal
Oct 26, 2011·The Journal of Experimental Medicine·Veit RothhammerThomas Korn
Apr 24, 2012·Trends in Immunology·Richa HanamsagarTammy Kielian
Jun 11, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sarah B SimmonsJoan M Goverman
Jun 15, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Joshua S StoolmanBenjamin M Segal
Jun 19, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yudong LiuHongwei Qin
Aug 5, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kevin S CarbajalBenjamin M Segal
Jan 24, 2016·The Journal of Investigative Dermatology·Jackson LiangYao LiLai Guan Ng
Dec 31, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Patrick C DunckerBenjamin M Segal

❮ Previous
Next ❯

Citations

Jan 8, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·William J Karpus
Jan 24, 2019·Journal of Clinical Medicine·Benjamin M Segal
Sep 26, 2020·Frontiers in Cellular Neuroscience·Melanie J PlastiniRoberta Brambilla
May 28, 2021·International Immunopharmacology·A Galán-SalinasL E Sánchez-Torres

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
FACS
flow cytometry

Software Mentioned

iQ5
aEAE
NIS Elements
FACS Diva
FlowJo
cEAE
Adobe Photoshop
GraphPad Prism
Treestar

Related Concepts

Related Feeds

Ataxia

Ataxia is a neurological condition characterized by lack of voluntary coordination of muscle movements including loss of coordination, balance, and speech. Discover the latest research on ataxia here.

Ataxias (MDS)

Ataxia is a neurological condition characterized by lack of voluntary coordination of muscle movements including loss of coordination, balance, and speech. Discover the latest research on ataxia here.

Ataxias

Ataxia is a neurological condition characterized by lack of voluntary coordination of muscle movements including loss of coordination, balance, and speech. Discover the latest research on different types of ataxias here.