PMID: 8970976Dec 1, 1996Paper

An IkappaB homolog encoded by African swine fever virus provides a novel mechanism for downregulation of proinflammatory cytokine responses in host macrophages

Journal of Virology
P P PowellRobert Michael Parkhouse

Abstract

Cytokines stimulate inflammatory defenses against viral infections. In order to evade host defenses, viruses have developed strategies to counteract antiviral cytokines. African swine fever virus (ASFV) is a large, double-stranded DNA virus that infects macrophages. This study demonstrates that ASFV effectively inhibited phorbol myristic acid-induced synthesis of antiviral, proinflammatory cytokines alpha interferon, tumor necrosis factor alpha, and interleukin-8 in infected macrophages as assessed by enzyme-linked immunosorbent assay and reverse transcriptase PCR. In contrast, levels of mRNA and protein for transforming growth factor beta, an anti-inflammatory cytokine, were increased by ASFV infection, suggesting that ASFV-induced inhibition of cytokine synthesis may be limited to cytokines activated by NFkappaB. An interleukin-8 promoter, containing an NFkappaB enhancer site, driving expression of a luciferase reporter gene was used to show that NFkappaB-dependent transcription was inhibited by the virus and by a cloned ASFV gene, A238L. This gene encodes a protein with homology to IkappaB, the inhibitor of NFkappaB. Electrophoretic mobility shift assay showed that cells expressing the A238L gene inhibited NFkappaB binding t...Continue Reading

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Citations

May 14, 2004·Journal of Virology·Trine H MogensenSøren R Paludan
Apr 13, 2015·Archives of Virology·Sílvia Cristina de Paiva e AlmeidaRobert Michael Evans Parkhouse
Aug 26, 1998·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·E AlbinaB Charley
Mar 22, 2002·Journal of Virology·Clara I RodríguezYolanda Revilla
Sep 20, 2000·The Journal of General Virology·S GoodbournR E Randall
Feb 14, 2004·Journal of Virology·Christelle Camus-BouclainvilleFrédérique Messud-Petit
Aug 29, 2009·PLoS Pathogens·Alfredo CastellóYolanda Revilla
Dec 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aitor G GranjaYolanda Revilla
Dec 20, 2007·The Journal of General Virology·Richard E Randall, Stephen Goodbourn
Jul 10, 2010·Journal of Biomedicine & Biotechnology·Gavin C Bowick, Alan D T Barrett
Sep 2, 1998·Immunology and Cell Biology·A Kelso
May 15, 1998·The Journal of Biological Chemistry·Y RevillaM Fresno
Oct 31, 2006·Oncogene·T D Gilmore, M Herscovitch
Mar 5, 2003·Pharmacological Reviews·Jan H von der ThüsenErik A L Biessen
Oct 29, 2010·Journal of Virology·Katharine Fagan-Garcia, Michele Barry
Nov 5, 2011·Cellular Microbiology·Gaëlle Le Negrate
Jan 17, 2008·The Journal of General Virology·David A G ChapmanLinda K Dixon
Jun 4, 2011·Bioengineered Bugs·Gavin C Bowick, Alexander J McAuley
Sep 17, 2013·The Veterinary Record·Chris Oura
May 24, 2005·Apoptosis : an International Journal on Programmed Cell Death·P ClarkeK L Tyler

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