An illustrated gardener's guide to transgenic Arabidopsis field experiments.

The New Phytologist
Martin FrenkelStefan Jansson

Abstract

Field studies with transgenic Arabidopsis lines have been performed over 8 yr, to better understand the influence that certain genes have on plant performance. Many (if not most) plant phenotypes cannot be observed under the near constant, low-stress conditions in growth chambers, making field experiments necessary. However, there are challenges in performing such experiments: permission must be obtained and regulations obeyed, the profound influence of uncontrollable biotic and abiotic factors has to be considered, and experimental design has to be strictly controlled. The aim here is to provide inspiration and guidelines for researchers who are not used to setting up such experiments, allowing others to learn from our mistakes. This is believed to be the first example of a 'manual' for field experiments with transgenic Arabidopsis plants. Many of the challenges encountered are common for all field experiments, and many researchers from ecological backgrounds are skilled in such methods. There is huge potential in combining the detailed mechanistic understanding of molecular biologists with ecologists' expertise in examining plant performance under field conditions, and it is suggested that more interdisciplinary collaboration...Continue Reading

References

Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z J ChenC S Pikaard
Apr 17, 2001·Trends in Plant Science·C L Ballaré
Jul 4, 2001·Journal of Experimental Botany·V BoudolfL De Veylder
Jul 18, 2001·Journal of Experimental Botany·M J Paul, C H Foyer
Jul 6, 2002·Science·Carsten KülheimStefan Jansson
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Jan 20, 2004·Plant Physiology·Ulrika GanetegStefan Jansson
Dec 15, 2004·Journal of Experimental Botany·A D B LeakeyM C Press

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Citations

Jan 10, 2013·PloS One·Hanna Johansson JänkänpääStefan Jansson
Apr 14, 2012·Plant, Cell & Environment·Hanna Johansson JänkänpääStefan Jansson
Nov 17, 2009·Molecular Ecology·Anne C DalzielPatricia M Schulte
Nov 19, 2010·Nature Reviews. Genetics·Joy Bergelson, Fabrice Roux
Jun 22, 2019·Journal of Experimental Botany·Catharina V HuberDietmar Funck
Oct 19, 2017·Ecology and Evolution·Unnikrishnan SivadasanRiitta Julkunen-Tiitto

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