An immune magnetic nano-assembly for specifically amplifying intercellular quorum sensing signals

Colloids and Surfaces. B, Biointerfaces
Yongguang GuanQin Wang

Abstract

Quorum sensing (QS) enables intercellular communication after bacterial cells sense the autoinducers have reached or exceeded a critical concentration. Selectively amplifying specific bacterial "quorum" activity at a lower cell density is still a challenge. Here, we propose a novel platform of immune magnetic nano-assembly to amplify specific bacterial QS signaling via improving the bioavailability of autoinducers-2 (AI-2, furanosyl borate) from sender (wide-type, WT cells) to receiver (reporter cells). Antibody coated magnetic nanoparticle (MNPAB) was fabricated with an average diameter of 12 nm and a specific surface area of 96.5 m2/g. The distribution efficiency of the antibody on the surface was 25.8 μg/m2 of magnetic nanoparticles. It was found that more than 3 × 108 of K12 serotype Escherichia coli (E. coli) reporter or WT cells were collected using 1 mg fabricated MNPAB at a saturated condition. The MNPAB not only captured E. coli WT cells but also brought them into proximity of E. coli (CT104, pCT6+pET-DsRed) reporter cells via magnetic attraction. The amplified QS signaling of the reporter cells by this immune magnetic nano-assembly was approximately 3 times higher than the nature QS signaling in cell suspension at opt...Continue Reading

References

Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·M G SuretteB L Bassler
May 7, 1999·Biosensors & Bioelectronics·I Abdel-HamidE Wilkins
Apr 4, 2002·Journal of the American Chemical Society·Chun-Cheng LinYi-Chun Wu
Oct 13, 2007·Journal of the American Chemical Society·Kheireddine El-BoubbouXuefei Huang
Nov 4, 2008·Current Opinion in Biotechnology·Sara Hooshangi, William E Bentley
Jul 1, 2009·Biotechnology Progress·Mona Utne LarsenNina C Shapley
Dec 22, 2009·Lab on a Chip·Jacob BartHan Gardeniers
Jun 1, 2010·Journal of the American Chemical Society·Rita L StrackRobert J Keenan
Sep 28, 2010·Environmental Science & Technology·Yan-Feng HuangXiu-Ping Yan
Jul 26, 2011·Journal of Hazardous Materials·Sarika SinghD Bahadur
Aug 8, 2012·Journal of the American Chemical Society·Kyoji TsuchikamaKim D Janda
Oct 12, 2012·Applied Microbiology and Biotechnology·Varnika RoyWilliam E Bentley
Jul 30, 2014·Nature Nanotechnology·Tanya GordonovWilliam E Bentley
Mar 25, 2015·Cell Reports·Jessica Ann ThompsonKarina Bivar Xavier
Jun 6, 2015·The ISME Journal·Matthew D ServinskyWilliam E Bentley
Oct 13, 2015·Nature Communications·Jessica L TerrellWilliam E Bentley
Jan 19, 2016·Journal of Biomedical Materials Research. Part a·Xiaoming LiFumio Watari

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